T细胞杀伤实验测试(T cell Killing Assay) 东大生物的T细胞杀伤测定法可鉴定受测品在促进或抑制T细胞活性后对靶肿瘤细胞杀伤/变态反应作用的影响程度。 这一方法可用于抗体受测品,如双特异性抗体(bscAb),特别是双特异性T细胞衔接子(BiTE)子类的抗体。 最终,将通过流式细胞术(FCM)通过活/死染料检查靶肿瘤细胞的生...
InnoModels Biotechnology 的 T 细胞介导的杀伤试验是一种具有巨大潜力的抗癌治疗方法。通过利用患者自身的T细胞,该检测能够有效杀死肿瘤细胞,为患者提供新的治疗选择。InnoModels Biotechnology将继续努力促进该检测在临床应用中的进一步发展。
Our study shows that T cells transduced with TCRs recognizing peptides from the cancer target TdT in the context of allogeneic HLA-A2 efficiently killed TdTposHLA-A2pos patient-derived lymphoblastic leukemia cells of B- and T-cell origin in vitro. While the killing efficacy mediated by TdT ...
In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a p... N Chaoul,C Fayolle,C Leclerc - BIO-PROTOCOL 被引量: 0发表: 2016年 in ...
In vitro T cell killing assay Purified OT-1 T cells were activated by anti-CD3 and anti-CD28 for 48 h. Purified DCs were loaded with 5 μg/ml OT-1 peptides for 30 min at 37 °C. After washed, DCs were coincubated with activated OT-1 T cells for 4 h at an E:T rat...
今天分享一个测试T细胞增殖的实验,可用于测试一些蛋白对于T细胞增殖的影响。 CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell ...
This invention regards the T cell response for the test antigen in regard to new T cell assay method, especially, with the removal of the controllable T cell rising. In order to make the detection of T cell response optimize, with the antigen or other sample the time when it cultures (...
图6:Luciferase Based Killing Assay检测原理图 评价CAR T细胞增殖活性 1. 免疫细胞代谢活性 (ATP 含量) 化学发光检测 ATP(三磷酸腺苷)是细胞活性的标志物,其仅存在于具有代谢活性的细胞内。由于萤光素酶和其底物 D-luciferin 反应需要ATP的存在才能产生光信号,其产生的化学发光信号同 ATP 含量成正比,因此可以通过...
Stable cell line generation To conduct the killing assay, target cells, including the K562, Raji, NCI-H441, p815 and A549, were transduced with a plasmid containing the firefly luciferase gene using the Sleeping Beauty transposon system [16]. For the generation of doxycycline (Dox)-inducible ...
11、Incucyte killing assay细胞杀伤试验 于96孔板中,在200ul不含IL-2的T细胞培养基中分别按照1:1,1:2,1:4,1:8的效应物:靶标(Effector:Target)的比例将5×10^4个表达GFP的肿瘤细胞与CAR-T细胞共培养,重复三份。使用Incucyte ZOOM Live-Cell (Essen Bioscience)分析系统,每2-6小时对培养板成像一次,持续...