This has sparked a deep interest in characterizing disease-associated T cell clonotypes as well as in identifying and quantifying their contribution to the pathophysiology of different autoimmune and inflammatio
T cell receptor (TCR) repertoire profiling As the field of immuno-oncology evolves, novel therapeutic strategies aimed at harnessing antitumor T cell cytotoxicity have shown promising results, from personalized neoantigen vaccines and oncolytic viruses, to immune checkpoint blockade (ICB) and adoptive cel...
Somatic mutations together with immunoediting drive extensive heterogeneity within non-small-cell lung cancer (NSCLC). Herein we examine heterogeneity of the T cell antigen receptor (TCR) repertoire. The number of TCR sequences selectively expanded in tumors varies within and between tumors and correlat...
Relevant features of T cell repertoire in human cancer remain to be delineated. Here the authors show, by TCR sequencing in a large cohort of lung cancer patients, that while a majority of T cell clones are shared between tumor and adjacent lung tissue, less frequent tumor-unique T cell clo...
Spatial resolution of the T cell repertoire is essential for deciphering cancer-associated immune dysfunction. Current spatially resolved transcriptomic technologies are unable to directly annotate T cell receptors (TCR). We present spatially resolved T cell receptor sequencing (SPTCR-seq), which integrate...
In this Tools of the Trade article, Ziwei Xue describes the development and use of scAtlasVAE, a deep learning framework that integrates single-cell RNA-sequencing data from CD8+ T cells across studies. Ziwei Xue Research Highlights02 Apr 2025 Nature Reviews Cancer Volume: 25, P: 319 Trea...
Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-ch
The pattern of responses suggests that many T cells in the gut repertoire recognize several bacterial strains from the community. We constructed T cell hybridomas from 92 T cell receptor (TCR) clonotypes; by screening every strain in the community against each hybridoma, we find that nearly all...
S. et al. T-cell receptor-alpha repertoire of CD8+ T cells following allogeneic stem cell transplantation using next-generation sequencing. Haematologica 104, 622–631 (2019). CAS PubMed PubMed Central Google Scholar Meyer, E. H. et al. A distinct evolution of the T-cell repertoire ...
Deep learning algorithms have been utilized to achieve enhanced performance in pattern-recognition tasks. The ability to learn complex patterns in data has tremendous implications in immunogenomics. T-cell receptor (TCR) sequencing assesses the diversity of the adaptive immune system and allows for mode...