solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot StartTaqDNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific...
SYBR Green Iis widely used in real-time PCR applications as an intercalating dye. SYBRGreenI是一种在实时定量PCR检测中广泛使用的DNA结合染料。 3) dye[英][daɪ] [美][daɪ] 染料染色染 4) dye[英][daɪ] [美][daɪ] 染色染料染 ...
solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot StartTaqDNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific...
The SYBR Green intercalating dye allows DNA detection and analysis without using sequence-specific probes. ROX serves as a passive reference dye.The use of Maxima SYBR Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, ...
Reaction specificity can be determined using a melting curve analysis, allowing you to identify non-specific qPCR products and primer-dimers. To perform a melting curve analysis, run a qPCR assay with a fluorescent intercalating dye like SYBR Green I. After amplification, the thermal cycler increase...
real-time PCRSYBR Green IIntroduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were...
The SYBR Green intercalating dye allows DNA detection and analysis without using sequence-specific probes. ROX serves as a passive reference dye.The use of Maxima SYBR Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, ...
SYBR Green I Dye-Based RQ-PCR Analysis The third possibility for RQ-PCR analysis is detection of PCR products via the DNA-intercalating dye SYBR Green I. This dye binds to the minor groove of double-stranded DNA, which greatly enhances its fluorescence. During the consecutive PCR cycles, the...
2.SYBR Green I is widely used in real-time PCR applications as an intercalating dye.SYBRGreenI是一种在实时定量PCR检测中广泛使用的DNA结合染料。 2)SYBR Green I dyeSYBRGreenI染料 3)Sybr Green I fluorescence quantitative reverse transcription polymerase chain reactionSybrGreenI荧光定量RT-PCR 4)SYBR...
Intercalating dyes were initially used to measureqPCRproducts (Higuchi et al., 1993). One of the most commonly used DNA intercalating dyes for qPCR today is SYBR® Green I. SYBR® Green I is a nonspecific fluorogenic minor groove DNA-binding dye that intercalates into double-stranded DNA...