Data was analysed using the clustering algorithm FlowSOM.Results: Mass cytometry allowed us to analyse and phenotype B cell subsets with unparalleled depth, revealing that three major subsets of CD24+ CD38 IgG3+ switched memory B cells were significantly elevated in active MS disease: CD21+ CD...
Switched memory B cells expressed more IL-6 and IL-12 mRNA than other subpopulations, but differences in respective protein levels were not significant. Moreover, we found that switched memory B cell transfer resulted in increased alveolar bone loss and periodontal osteoclastogenesis. Moreover, ...
The mechanisms of secondary changes in antibody repertoires remain unclear. McHeyzer-Williams and colleagues define the stages of reentry of class-switched memory B cells into germinal centers to remodel existing antibody specificities. Effective vaccine
We conclude that the presence of intact ITT motifs in the cytoplasmic domains of membrane-bound IgG molecules improves the lifespan of IgG- switched memory B cells, presumably by enhanced tonic signal- ling of ITT-containing mIgG–BCRs23. ITT signalling promotes production of IgG antibodies. To...
After primary vaccination Down syndrome children generate significantly less specific switched memory B cells than their siblings. The response to a booster dose of vaccine is instead comparable in both groups. The production of specific antibodies was equally effective in Down syndrome and controls ...
The negative correlation between B cells and Tregs in blood is in line with previously published observations that Tregs can suppress B cells. Future studies focusing on the presence of these (class switched) memory B cells in the lung, their antigen specificity and their interaction with Tregs ...
Flow cytometric analysis of peripheral blood lymphocytes revealed a reduction of class-switched CD27(+)IgM(-)IgD(-) memory B cells below 0.4% in 77% of our patients (group I), while this B-cell subpopulation exceeded 0.5% in all healthy donors and in 23% of CVID patients (group II)....
Memory B cells express high-affinity, immunoglobulin GB cell receptors (IgG BCRs) that enhance B cell responses, giving rise to the rapid production of high-affinity, IgG antibodies. Despite the central role of IgG BCRs in memory responses, the mechanisms by which the IgG BCRs function to ...
Maturation of the antibody response entails B cell somatic hypermutation (SHM), class-switch DNA recombination (CSR), differentiation of plasma cells (PCs) making high-affinity antibodies and generation of specific memory B cells (MBCs). The National Institute of Allergy and Infectious Diseases has ...
This dependence on transistor matching can be avoided by using current memory cells. As depicted in FIG. 1, a current memory cell 11, also known as a current copier or dynamic current mirror, should be capable of making an accurate copy of an input current, Iin, during a sample phase of...