Keywords: non-coding sequence; GFP; stem-loop; Alu; SV40PolyA 真核和原核生物基因组中均存在非编码序列,真核生物基因组中非编码序列较多,原核 生物基因组中非编码序列较少,如人基因组中大于 95% 的序列属于非编码序列,而大肠杆 菌基因组中非编码序列约占 11%。非编码序列中蕴藏着重要的有关遗传和发育的...
关键词: Alu; SV40 PolyA; AATAAA 信号; GFP; 转录终止 The effects of SV40 PolyA sequence and its AATAAA signal on up-stream GFP gene expression and transcription termination LI Shu-Ping 1, 2 , FENG Jing-Jing 3 , WANG Hong-Gang 4 , WANG Xiu-Fang 1 , LV Zhan-Jun 1 1. Department of...
关键词:Alu;SV40PolyA;AATAAA信号;GFP;转录终止TheeffectsofSV40PolyAsequenceanditsAATAAAsignalonup-streamGFPgeneexpressionandtranscriptionterminationLIShu-Ping1,2,FENGJing-Jing3,WANGHong-Gang4,WANGXiu-Fang1,LVZhan-Jun11.DepartmentofGenetics,HebeiKeyLabofLaboratoryAnimal,HebeiMedicalUniversity,Shijiazhuang050017,...
SV40PolyAOur previous studies showed that tandem Alu repeats inhibited GFP gene expression when they were inserted into the downstream of GFP gene in pEGFP-CI vector and HeLa cells were then transfected transiently.The sequence named 2F2R (second 60 bp from the 5' end of SV40PolyA antisense ...
关键词:非编码序列;L1重复序列;Alu重复序列: SV40PolyA;荧光阳性蛋白;基因表达调控 英文摘要 Both candecreasethe of and SV40PolyAPolyAas inhibitionofGFP caused expressionbyL1,Alu insertion ABSTRACT DNA inhuman Objectives:98%of sequence genome to themale belongs of of non-codingsequence.Halfcomprised the ...
环结构均不利于活化基因，提示适当的不完整茎环结构与活化基因有关。关键词：非编码序列；GFP；茎环结构；Alu；SV40PolyA Detection and sequence analysis of an imperfect stem-loop structure in cis activating gene element from SV40PolyA WANG Hong-Gang1,2, MA Huan1, LI Zhu3, ZHANG Bin3, JING Xiang-...
茎环; 互补性; GFP; 转染 关键词:SV40PolyA; 英文摘要 Effectofmutationsof elementin Oil activating SV40PolyA gene GFP reportergeneexpression ABSTRACT area lotof inhumanand objective:Therenon.codingsequences functionofmost mammalian iSnotclearaboutthe non—coding genome.It thenew researchesofthe mechanism...
SV40 PolyA(猴空泡病毒PolyA, 简称PolyA)序列是有转录终止作用和使转录的mRNA 添加PolyA 尾的 DNA 序列(240 bp), 含有AATAAA 六核苷酸多腺苷化信号(Polyadenylation signal)。 AATAAA六核苷酸是最早被认识到的mRNA多腺苷化信号[1], 90%的真核mRNA 的AATAAA 序列都在距剪切位点的30 个核苷酸的范围内[2], 它指导...
另一个的特征是在猪抑肌素3'UTR有两个polyA信号序列(AATAAA).进而,我们在进化保守区域分析到许多潜在的转录因子结合位点,它们可能与抑肌素的调控有关.许多潜在的... 于政权,李岩,孟庆勇,... - 《中国科学》 被引量: 17发表: 2004年 [The effects of SV40 PolyA sequence and its AATAAA signal on upstream...