SV40 Large Tumor Antigen Nuclear Import Is Regulated by the Double-stranded DNA-dependent Protein Kinase Site (Serine 120) Flanking the Nuclear Localization SequencefatsdeuteriumconjugatedcistransisomersNuclear localization sequence (NLS)-dependent nuclear import of SV40 large tumor antigen (T-Ag) fusion ...
SV40 DNA replication requires interactions between proteins provided by the host cell, the virus-coded A protein and a viral sequence of 80 base pairs (bp)... M Lusky,M Botchan - 《Nature》 被引量: 980发表: 1981年 Nuclear targeting of SV40 and adenovirus Import o f viral DNA into the ...
Factors Influencing the Ability of Nuclear Localization Sequence Peptides To Enhance Nonviral Gene Delivery Nonviral gene delivery is limited by inefficient transfer of DNA from the cytoplasm to the nucleus. Nuclear localization sequence (NLS) peptides have been ... KH Bremner,LW Seymour,A Logan,...
For direct comparison with the short peptide, we prepared a long peptide containing the NLS and its flanking sequences of SV40 large T-antigen and its mutated long peptide, in which possible phosphorylation sites located at the amino terminal of the NLS were changed to alanine. Kinetic ...
The data suggest that the use of NLS may reduce the need for using elevated DNA copy numbers in some gene transfer applications.doi:10.1007/BF01980210Philippe CollasHarald HusebyePeter AlestrmTransgen ResCollas, P., Husebye, H., Alestrom, P. 1996 The nuclear localization sequence of ...
The amino acid sequence homologous to SV40 T NLS is an immunologically active epitope on the Ki antigen; reactivity to this epitope is associated with characteristic clinical features allied with anti-Ki antibodies in lupus patients.doi:10.3109/s101650200008T. Yano...
14-3-3 Protein interacts with nuclear localization sequence of forkhead transcription factor FoxO4. According to our best knowledge, there is no experimental evidence showing a direct interaction between the 14-3-3 protein and NLS of FoxO. Therefore, ... V Obsilova,J Vecer,P Herman,... -...
Proteins larger than 45 kDa require a nuclear localization sequence (NLS) (4) to be targeted to the nucleus. NLSs are defined as the sequences sufficient and necessary for nuclear import of their respective proteins (5, 6, 7) and are generally functional in targeting heterologous, normally ...