The translation of PTENβ is reported to be in-frame with the AUG start sequence of a conventional PTEN. PTENβ is mostly found in the nucleolus, where it directly interacts with and removes phosphate groups from nuciferin. Disruption of PTENβ results in an abnormal rise in pre-rRNA ...
Human codon-optimized LtrA protein (hLtrA) with an SV40-NLS expressed in human cells has reverse transcriptase activity and localizes to the nucleus.David M. TruongF. Curtis HewittJoseph H. HansonXiaoxia CuiAlan M. Lambowitz
The comparison of eye size is underlined with a red frame in the lateral view and with diameter measurement in the dorsal view. Red arrows indicate a pointy head and slight pericardial oedema of tsr2 (M1V)/− mutants. Scale bar: 500 μm. This experiment was repeated three times ...
we introduced a linker sequence containing DraI- SphI- BlpI 41 bps site in frame with the CjCas9 protein. The resulting plasmid, pBK1294a, has been used as a common intermediate for the following
NLS: Nuclear Localization signals NTAR: N-Terminal Activating Region ORF: Open Reading Frame PI3K: Phosphatidylinositol 3-kinase PLCγ1: Phospholipase C gamma 1 PP2A: Phosphatase 2 A pRb: Retinoblastoma protein Pur-alpha: Purine-rich element binding protein A ...
In this frame, DOPAL has been demonstrated to covalently modify αSyn lysines triggering its aggregation into SDS-resistant annular-shaped off-pathway oligomers [11,12,13]. DOPAL-induced αSyn oligomeric species are more resistant to degradation and their aberrant accumulation at the pre-synaptic ...
However, integration occurs long before cancer becomes manifest [129,130], and is also seen with the noncancerogenic DHBV [131]. Hence integration appears to be a common consequence of hepadnavirus infection; as hepadnaviral genomes lack an integrase-like open reading frame integration must be...
Within the time frame of this experiment (1 hr incubation), the pre-mRNA containing both introns is no longer detected, but in addition to the final mRNA product and the intron lariats a splicing intermediate containing one remaining intron is still detectable. Interestingly, the anti-Y14 ...
To express C-terminal translational fusions by means of our promoters, tags and destination vectors, entry vectors containing the GOI should be cloned with a start codon and without a stop codon, and the manufacturer’s guidelines regarding the reading frame should be adopted (http://www.invitrog...
First, to further enhance CasRx activity, we tested all five combinations of CasRx N- and C-terminal fusions to the SV40 NLS used in CasRx or to a bpNLS, then assessed the ability of the five CasRx systems to knock down mCherry protein levels in N2A cells using pools of four pre-...