Subhashini Pon, Raja S, Thangaradjou T. Establishment of cell suspension culture protocol for a seagrass (Halodule pinifolia): Growth kinetics and histomorphological characterization. Aquat Bot. 2014; 117:33-40.Subhashini P, Raja S, Thangaradjou T. Establishment of cell suspension culture protocol...
Protocol for Passaging Suspension Cells Video: Passaging cells This video explains why, when and how to passage cells grown in both adherent and suspension cultures. This includes cell dissociation, counting cells, determining optimal seeding density and preparing new culture v...
The following protocol describes a general procedure for passaging mammalian cells grown in suspension culture using shaker flasks in a shaking incubator.For detailed protocols, always refer to the cell-specific product insert. Note:Make sure that the shaker flask does not ...
This study demonstrates nearly 100-fold iPSC expansion over 5-days using our 3D suspension culture protocol in Vertical-Wheel® bioreactors, the largest cell growth reported to date. 3D expanded cells showed enhanced in vitro and in vivo pluripotency phenotype that may support more efficient scale...
, such a protocol would be a monumental contribution to the biotechnological utility of cannabis and other medicinal plants (Bioharvest, 2021). The application of artificial compartmentalization is another promising strategy for avoiding toxicity in cannabis cell suspension culture (Andre et al., 2016)...
About this protocol Cite this protocol Moscatiello, R., Baldan, B., Navazio, L. (2013). Plant Cell Suspension Cultures. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_5 ...
The protocol described here is a validated method for recovery of physiologically relevant amounts of key metabolites from suspension-cultured mammalian cells. The method is a two-step process consisting of initial quenching of the cells (to stop cellular metabolism and allow isolation of the cells)...
The commonly used methods of initiation of a callus from a plant and subsequent steps from callus to cell suspension culture are presented in the protocol. This is followed by three different techniques for subculturing (by weighing cells, pipetting and pouring cell suspension) and four methods ...
A protocol for regeneration of tetraploid tall fescue (Festuca arundinacea var. glaucescens 2n = 4x = 28) from cell suspension cultures is described. Calluses were induced from the basal part of youngest leaves and used for initiation of suspensioncultures. Fast growing cell suspension cultures were...
The following protocol describes a general procedure for passaging mammalian cells grown in suspension culture using shaker flasks in a shaking incubator. For detailed protocols, always refer to the cell-specific product insert. Note: Make sure that the shaker flask does not...