Herein, we present a modified iPSC expansion protocol using 3D suspension culture within Vertical-Wheel® bioreactors that achieves the largest cell expansion to date, in a single passage, while maintaining a high-quality cell product. We evaluate the expansion potential of this 3D suspension protoc...
Cell culture vessels Mix and store your suspension cultures in the same vessel with these economical, lightweight, and break-resistant Nalgene culture vessels. Related products Gibco Bioprocessing Whether you are performing cell culture in a research lab or in a production facili...
The commonly used methods of initiation of a callus from a plant and subsequent steps from callus to cell suspension culture are presented in the protocol. This is followed by three different techniques for subculturing (by weighing cells, pipetting and pouring cell suspension) and four methods ...
Most cells are anchorage- dependent and must be cultured while attached to a solid or semi-solid substrate (adherent or monolayer culture), while others can be grown floating in the culture medium (suspension culture). Cryopreservation If a surplus of cells are available from subculturing, they ...
5.1 Overview Cells grown in culture are harvested and washed. 5.2 Duration 20 min 1.1 Collect cells in a 15-ml conical centrifuge tube. If starting with tissue culture cells in suspension, directly collect suspension in a conical tube. If starting with adherent cells in culture, trypsinize and...
artificial seawater (CMF)4prior to counting. Cell concentrations at time point zero were calculated by counting the cell suspension and then resuspending in either ASW, M199, or M1 to the desired concentration. Samples were cultured in 24-well plates (Falcon): for the first two experiments (...
Dr. Ronaldson-Bouchard explains why adapting cell-based assays from suspension cells to 2D culture, 3D culture, to ‘patient in a dish’ systems is not an exercise undertaken lightly, however, the effort pays off. Learn More Simplifying Immune Oncology Research ...
Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture. Biomaterials. 1996;17:879–88. Article CAS PubMed Google Scholar Luo X, Liu Y, Pang J, Bi S, Zhou Z, Lu Z, et al. Thermo/photo dual-crosslinking chitosan-gelatin methacrylate hydrogel with ...
Ascorbic acid (AA) has been employed in various cell culture conditions for its ability to suppress the generation of reactive oxygen species1,2. Particularly, AA has been shown to exert beneficial effects in stem cell culture. For example, the compound enhances the generation of induced pluripote...
Hink, W.F. 1982. Production ofAutographa Californianuclear polyhedrosis virus in cells from large-scale suspension cultures, 493–506.In:Microbial and Viral Pesticides. Kurstak, E. (Ed.), Marcel Dekker, Inc. Google Scholar Vaughn, J.L. 1976. Production of NPV in large-scale cultures.J. In...