human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by an OCT4+/TRA1-60/TRA1-81/SSEA1+/SSEA4 expression signature, and novel methods of generating and maintaining same in a pluripotent, undifferentiated state a suspension culture devoid of cell clumps....
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MSC1 at passage 3, and MSC2 at passage 1, were prepared for scRNA-sequencing assays. MSCs were digested into a single-cell suspension, and the cell density and live cell proportion were detected using a Countess II Automated Cell Counter. MSC samples with a density of 1000 cells ...
Up-scaling single cell-inoculated suspension culture of human embryonic stem cells We have systematically developed single cell-inoculated suspension cultures of human embryonic stem cells (hESC) in defined media. Cell survival was depend... H Singh,P Mok,T Balakrishnan,... - 《Stem Cell ...
1999. Cultured human keratino- cytes as a single cell suspension in fibrin glue combined with pre- served dermal grafts enhance skin reconstitution in athymic mice full-thickness wounds. Eur J Plast Surg 22:237.b. Horch, R.E., Bannasch, H., and Stark, G.B. Cultured human keratinocytes ...
Next, we derived a cell line named ssf-MDCK, which was amenable for single-cell suspension culture in the serum-free medium. We found that during serum-free batch culture of MDCK cells, the peak viable cell density and maximum specific growth rate were 3.81×106 cells/mL and 0.056 h?1,...
英文名称:Single Cell Suspension Solution 纯度:99 % 产地:中国/上海 品牌:泽叶生物 包装规格:1 mL 货号:ZY130805 别名:单细胞悬浮液 单细胞悬浮液(Single Cell Suspension Solution)提供了更简单、更快速和更高质量的方法,包括质粒提取、胶回收、PCR产物纯化、基因组提取、RNA分离纯化、PCR/RT-PCR、荧光定量、...
annotated cells22(Fig.1b). We subsampled the datasets before downstream integration to equalize group sizes and reduce unwanted variability using multiple stratifications (Methods). This resulted in a more balanced distribution of cell types, donors and nuclei and amplified rarer cell type populations...
First, we digested third-passage of FSMSCs and HuMSCs with cell survival rate higher than 80%. Second, the concentration of cell suspension was adjusted to 1 × 106cells/mL for the 10× Genomics Chromium platform (Single Cell 3′ library and Gel Bead Kit V3). The cell sequencing cou...
The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perf