3.1. MSC CellSaic相关的细胞治疗研究 在MSC CellSaic相关的疾病治疗研究中,有MSC CellSaic应用于IBD (炎症性肠病)、脑梗塞、关节的骨软骨再生、胰岛移植的报告。这些应用的研究中,与单独使用MSC进行比较,使用MSC CellSaic 可以提高各种症状的改善效果。 在DSS模式小鼠的炎性肠病的适用研讨中,单独使用MSC而症状没...
Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it has been demonstrated that the production of vari...
在MSC CellSaic相关的疾病治疗研究中,有MSC CellSaic应用于IBD (炎症性肠病)、脑梗塞、关节的骨软骨再生、胰岛移植的报告。这些应用的研究中,与单独使用MSC进行比较,使用MSC CellSaic 可以提高各种症状的改善效果。 在DSS模式小鼠的炎性肠病的适用研讨中,单独使用MSC而症状没有得到充分改善的模式小鼠,通过给药MSC ...
Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it
Cells were resuspended in 100 µL buffer, a sample was obtained to assess baseline MSC:PBMC ratio by flow cytometry, then 10 µL/10 million cells of human anti-PE nanobeads from the selection kit was added to the remaining cell suspension, and the cells were incubated on ice for 15 ...
Due to the limited downstream processing steps and shelf-life requirements for cell therapies, single use technologies are essential for cell therapy production. However, typical single-use static in vitro culture methods, are often too cumbersome and inefficient to support commercial scale production ...
Cells were resuspended in 100 µL buffer, a sample was obtained to assess baseline MSC:PBMC ratio by flow cytometry, then 10 µL/10 million cells of human anti-PE nanobeads from the selection kit was added to the remaining cell suspension, and the cells were incubated on ice for 15 ...
Mesenchymal Stem/Stromal Cells (MSC) are a promising cell type for cell-based therapies - from tissue regeneration to treatment of autoimmune diseases - due to their capacity to migrate to damaged tissues, to differentiate in different lineages and to th
were digested, harvested and resuspended in PBS at a concentration of 3 ×105/mL; 500 μL 0.75 % normal-melting-point agarose was coated on slides acting as the first layer; 100 μL 0.7 % low-melting agarose mixed with 30 μL cell suspension was spread on the first ...
The bone marrow was repeatedly washed to generate a single-cell suspension that was centrifuged at 1000 rpm for 5 min. The supernatant was removed, and cells were washed with DMEM/F12 and centrifuged for an additional 5 min. Finally, the supernatant was removed, and cells were ...