human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by an OCT4+/TRA1-60/TRA1-81/SSEA1+/SSEA4 expression signature, and novel methods of generating and maintaining same in a pluripotent, undifferentiated state a suspension culture devoid of cell clumps....
英文名称:Single Cell Suspension Solution 纯度:99 % 产地:中国/上海 品牌:泽叶生物 包装规格:1 mL 货号:ZY130805 别名:单细胞悬浮液 单细胞悬浮液(Single Cell Suspension Solution)提供了更简单、更快速和更高质量的方法,包括质粒提取、胶回收、PCR产物纯化、基因组提取、RNA分离纯化、PCR/RT-PCR、荧光定量、...
1999. Cultured human keratino- cytes as a single cell suspension in fibrin glue combined with pre- served dermal grafts enhance skin reconstitution in athymic mice full-thickness wounds. Eur J Plast Surg 22:237.b. Horch, R.E., Bannasch, H., and Stark, G.B. Cultured human keratinocytes ...
Up-scaling single cell-inoculated suspension culture of human embryonic stem cells We have systematically developed single cell-inoculated suspension cultures of human embryonic stem cells (hESC) in defined media. Cell survival was depend... H Singh,P Mok,T Balakrishnan,... - 《Stem Cell ...
Paul, J. 1970.Cell and Tissue Culture. 4th Ed. Livingstone, Edinburgh, pp. 215–218. Google Scholar Burger, M. M. 1970. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth. Nature 227: 170–171. ...
Following preparation of the cell suspension, the HSCs were further isolated using a Nycodenz density gradient. Cell viability was examined by trypan blue staining, and the purity of the quiescent human HSCs was determined by autofluorescence and oil red O staining. Activated and quiescent human ...
The single-cell suspension was then pelleted and washed once in MACS buffer. Density-gradient centrifugation using a three-layer Percoll gradient (GE Healthcare) with specific gravities of 1.115, 1.065, and 1.0 was used to enrich for stromal cells. Cells isolated from the Percoll-light fraction...
notes that cultured cell lines can readily be transformed into a single-cell suspension either by gentle agitation with a pipette to break up small clumps of suspension cells and dissociate loosely adherent cells, or by dislocation from the culture plate or flask using an appropriat...
A suspension of cells from culture (or peripheral blood) is deposited on custom-made arrays containing micro-wells, controlling distribution and density to deposit one cell in each well. The array is then screened by a process known as micro-engraving—the array is covered with a glass slide ...
Preparation of single-cell suspension Each tissue sample was transferred to a sterile 10-cm culture dish and cut into < 1 mm3 segments before being transferred to a 50-ml centrifuge tube. Each tissue type was then digested in the DMEM (Sigma-Aldrich, USA) containing 100 μg/ml collagenase ...