transmembrane proteinmyc epitopesurface expressionexpression differenceIn many cases the expression of a soluble protein on the cell surface is desirable for the study of a functional protein, more applications of a protein, or investigation of protein–protein interaction. The expression of a soluble ...
A second class oftransmembrane receptorsare the kinase receptors. These proteins typically contain an extracellular ligand-binding domain at the amino terminus, a single membrane-spanning domain, and an intracellular carboxy-terminus that includes the kinase domain. In most cases, binding of the lig...
Specific transmembrane proteins are sorted to the magnetosome membrane (MM). MamC is the most abundant MM protein of Magnetospirillum gryphiswaldense strain MSR-1. MamF is the second most abundant MM protein of MSR-1 and forms stable oligomers. We expressed staphylococcal protein A (SPA), an...
Qualitative analysis of build-up curves obtained for different mixing times allowed the transmembrane peptide in PC10 to be distinguished from the surface bound topology in POPC. The 13 C-MAOSS results thus independently confirms previous findings from 15 N spectroscopy [Bechinger, B., Skladnev, ...
Multinucleated osteoclasts, essential for skeletal remodeling in health and disease, are formed by the fusion of osteoclast precursors, where each fusion event raises their bone-resorbing activity. Here we show that the nuclear RNA chaperone, La protein has an additional function as an osteoclast fus...
results in “self-labeling” of the cells upon binding of the tagged molecules to the target protein15. However, a potential issue with this system is the occurrence of cross-labeling or binding of secreted molecules to the surface of another cell....
Teneurins, a transmembrane protein family involved in cell communication during neuronal development The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation An Innovative Method to Identify Autoantigens Expressed on the Endothelial Cell Surface Serological Ide...
The invention also concerns the uses of said antibodies for selecting cells expressing at their surface said protein. Said isolated protein has a structure (a) comprising an extracellular domain located between positions 21 and 152, five transmembrane domains located between positions 153 and 295 and...
These experiments provide the first evidence for the existence of a transmembrane pore, analogous to the pore of ion channels, for this new type of cell-surface receptor. 展开 关键词: Animals Cercopithecus aethiops Humans COS Cells Vitamin A Membrane Proteins Amino Acid Substitution Protein Structure...
G-protein-coupled receptors (GPCR) are a superfamily of seven transmembrane proteins acting as signal transducers that activate different downstream signaling pathways. They are divided into 6 classes: class A (rhodopsin-like), class B (secretin receptor family), class C (metabotropic glutamate/pherom...