transmembrane proteinmyc epitopesurface expressionexpression differenceIn many cases the expression of a soluble protein on the cell surface is desirable for the study of a functional protein, more applications of a protein, or investigation of protein–protein interaction. The expression of a soluble ...
The surface of Trypanosoma brucei is dominated by glycosyl-phosphatidylinositol (GPI)-anchored proteins, and endocytosis is clathrin dependent. The vast majority of internalized GPI-anchored protein is efficiently recycled, while the processes by which transmembrane domain (TMD) proteins are internalized an...
Control of Drosophila gastrulation by apical localization of adherens junctions and RhoGEF2. Science 315, 384–386 (2007). Identifies the transmembrane protein T48 that promotes D. melanogaster mesoderm invagination by recruiting the cytoskeletal regulator RhoGEF2 to the sites of apical constriction. ...
Modular arrangement of extracellular regions and protein superfamilies The extracellular regions of immune cell surface proteins include distinct domains which are often connected to the transmembrane domain by a linker or stalk region. Only one or two domains may have specific binding functions. Others ...
Multinucleated osteoclasts, essential for skeletal remodeling in health and disease, are formed by the fusion of osteoclast precursors, where each fusion event raises their bone-resorbing activity. Here we show that the nuclear RNA chaperone, La protein has an additional function as an osteoclast fus...
Qualitative analysis of build-up curves obtained for different mixing times allowed the transmembrane peptide in PC10 to be distinguished from the surface bound topology in POPC. The 13 C-MAOSS results thus independently confirms previous findings from 15 N spectroscopy [Bechinger, B., Skladnev, ...
ADAMs cleave a variety of transmembrane proteins, including APP, within their extracellular domains, resulting in shedding of the ectodomain fragment [4]. Multiple receptor ligands, as well as direct activators of protein kinase C (PKC), stimulate ectodomain shedding of APP and other proteins [6]...
These experiments provide the first evidence for the existence of a transmembrane pore, analogous to the pore of ion channels, for this new type of cell-surface receptor. 展开 关键词: Animals Cercopithecus aethiops Humans COS Cells Vitamin A Membrane Proteins Amino Acid Substitution Protein Structure...
results in “self-labeling” of the cells upon binding of the tagged molecules to the target protein15. However, a potential issue with this system is the occurrence of cross-labeling or binding of secreted molecules to the surface of another cell....
G-protein-coupled receptors (GPCR) are a superfamily of seven transmembrane proteins acting as signal transducers that activate different downstream signaling pathways. They are divided into 6 classes: class A (rhodopsin-like), class B (secretin receptor family), class C (metabotropic glutamate/pherom...