transmembrane proteins have been created to accurately fold into designed structures [4–6], to transport ions and small molecules across the membrane [7–10], to specifically interact with target transmembrane helices [11], to bind small-molecule cofactors [12,13] and to fine-tune cell ...
Post-mitotic reassembly of nuclear envelope (NE) and the endoplasmic reticulum (ER) has been reconstituted in a cell-free system based on interphase Xenopus egg extract. To evaluate the relative contributions of cytosolic and transmembrane proteins in NE and ER assembly, we replaced a part of ...
Tetraspanins are a superfamily of membrane proteins which span the membrane four times; they are found predominantly at the cell surface but are also located on intracellular vesicles. Tetraspanins (with a few exceptions) do not have con... Palmer,John 被引量: 0发表: 2016年 Mast cells and th...
Cell membrane spanning proteins contain a luminal/extracellular domain, a transmembrane region and a cytosolic domain. In a type I transmembrane protein the N‐terminus is the extracellular/luminal part of the protein, whereas the C‐terminus comprises the cytosolic region of the membrane protein. Tu...
MembraneproteinTemperaturesensingTransmembranesignalingLipidinteractionCell membranes are composed of a lipid bilayer containing proteins that cross and/or interact with lipids on either side of the two leaflets. The basic structure of cell membranes is this bilayer, composed of two opposing lipid ...
Liquid-liquid phase separation of proteins occurs on both surfaces of cellular membranes during diverse physiological processes. In vitro reconstitution could provide insight into the mechanisms underlying these events. However, most existing reconstitut
An important subfamily of membrane proteins are the transmembrane α-helical proteins, in which the membrane-spanning regions are made up of α-helices. Given the obvious biological and medical significance of these proteins, it is of tremendous practica
Alpha-helical transmembrane (TM) proteins are involved in a wide range of important biological processes such as cell signaling, transport of membrane-impermeable molecules, cell-cell communication, cell recognition and cell adhesion. Many are also prime
The proteins SIDT1 and SIDT2 share identity and conserved cholesterol binding (CRAC) domains with C. elegans ChUP-1. Both mammalian proteins are annotated as RNA transporters in databases. In the present study, we show evidence indicating that SIDT1 and SIDT2 not only do not transport RNA, ...
A targeted mass spectrometry-based method is presented that adopts the fast photochemical oxidation of proteins (FPOP) for footprinting of cystic fibrosis transmembrane conductance regulator (CFTR) membrane transporter at its original plasma membrane location. Two analytical imperatives were sought: (1) ...