The advantage of using such systems is that the viruses contain few structural proteins, including usually one (VSV:G protein) or two (Sindbis: E1 and E2) integral membrane glycoproteins and in some cases, an internal peripheral membrane protein (VSV:M protein). In contrast, an average ...
2. The first use of a therapeutic protein developed was insulin, used as a therapy for diabetes mellitus3. Since then, over 200 protein-based compounds have been approved for clinical use and over 250 proteins are currently in various stages of clinical evaluation4,5. While...
To test the relative functional importance of the NPC’s central and peripheral channels in nuclear translocation of transmembrane proteins, we performed experiments imaging the nuclear transport of LBR-N after disrupting either central or peripheral channel transport. To block the central channel we mic...
Staining was present in the cytoplasm but there was often also stronger peripheral labeling in the region of the cell membrane. Table 2. Immunostaining of normal hematopoietic tissues for transmembrane adaptor proteins Empty CellLATLIMETRIMNTALPAGSIT Tonsil/lymph node Lymphoid follicles Germinal ...
Characterization of the envelope transmembrane protein of human immunodeficiency virus type 2 (HIV-2) was carried out using murine polyclonal and monoclonal antibodies or patient sera specific for HIV-2 proteins. A 80-Mr glycoprotein (gp80) was produced in HIV-2 infected cells along with three ot...
(There has been some success with L-DOPA treatment in Parkinson's patients, and multiple sclerosis can be treated with cytokines acting on the peripheral immune system.) (See generally, Partridge, supra). In many of the above listed disorders, delivery across the BBB is the rate limiting ...
Transmembrane Membrane Readers form a Novel Class of Proteins That Include Peripheral Phosphoinositide Recognition Domains and Viral Spikes. Membranes 2022, 12, 1161. [Google Scholar] [CrossRef] [PubMed] de Planque, M.R.; Bonev, B.B.; Demmers, J.A.; Greathouse, D.V.; Koeppe, R.E.,...
This rapid and cost-effective approach enabled the identification of antigen-specific B cells in the peripheral blood of patients suffering from severe COVID-19. After that, distinct BCRs were extracted, replicated, and manufactured into complete antibodies. Their reaction to the spike RBD domain ...
To identify baseline peripheral temperature indices (PI) before anesthesia that predict a decrease in central temperature 30 minutes after anesthesia induction, and the rate of change in PI predicting the decrease in central temperature 60 minutes after induction, a receiver operating characteristic (ROC...
The goal of the mini-review is to examine the data on annexin-lipid interactions from the last ~30years from the point of view of the controversy between the two lines of inquiry: the well-characterized peripheral assembly of the annexins at membranes vs. their putative transmembrane insertion...