Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli[J]. Journal of Virology, 1989, 63 (12):5037-5045. [47]Wang QM, Chen SH. Human rhinovirus 3C protease as a potential target for the development of antiviral agents[J]. Current Protein ...
Use SUMO Protease to cleave SUMO, resulting in the production of native protein with no extra amino acids added between the cleavage site and the start of your protein. Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center. ATG is often sufficient ...
The SUMOstar™ tag remains tethered to the protein throughout the purification process. Precise C-terminal removal of the tag simply requires end user-induced cleavage by engineered SUMOstar™ proteases. The native N-terminal residues of the protein are completely preserved following cleavage, ...
Coexpression of these two proteins results in the in vivo cleavage of the protein of interest from the SUMO tag, while still leaving the protein of interest in a form that can be purified from a soluble cell lysate by affinity chromatography. 展开 ...
MS/MS analyses of these branch peptides generally reveal abundant fragment ions resulting from cleavage of the SUMO tail, but which obscure those needed for characterizing the target peptide sequence. Other approaches for identifying SUMO substrates exist and include overexpression of the SUMO isoforms ...
K. Phosphorylation-dependent interaction of SATB1 and PIAS1 directs SUMO-regulated caspase cleavage of SATB1. Mol. Cell. Biol. 30, 2823–2836 (2010). Article CAS PubMed PubMed Central Google Scholar Okubo, S. et al. NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its...
Lanes alternate with (+) and without (−) Ulp1(403–621)p in the reaction. Lanes 1 and 2 (SDS-PAGE): cleavage reactions with a C-terminally His-tagged human Sumo-1 protein. Lanes 3 and 4 (SDS-PAGE): cleavage reactions with a His6-ubiquitin-Smt3-HA fusion protein. Lanes 5 and ...
3Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA Discovery of emerging REGγ-regulated proteins has accentuated the REGγ-proteasome as an important pathway in multiple biological processes, including cell growth, cell cycle regulation...
Coli. ? Our three-step purification procedure can be applied to a wide variety of targets. ? We obtain pure SUMO-1 modified IκBα in milligrams quantities. ? High yield expression is suitable for structural studies. 展开 关键词: Sumoylation IκBα NF-κB On-column tag cleavage ...
Moreover, the long SUMO branch left on modified peptides after trypsin cleavage gives rise to tandem MS (MS/MS) spectra that are difficult to interpret32. Here we use a newly designed two-step enrichment strategy that overcomes these obstacles and permits site-specific identification of SUMO ...