is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO protease itself has a His tag for easy removal from the protein ...
A new SUMO-specific affinity tag is described herein, based on the amino acid sequence from 403-621 of the SUMO protease Ulp1, and containing a crucial substitution of serine for cysteine. This affinity tag is particularly useful for a range of applications, including detection and affinity ...
Greatly enhanced solubility with an N-terminal SUMO fusionHighly efficient cleavage- produces native protein of interest with SUMO (ULP-1) protease*Highly specific cleavage- eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificantly incr...
Theoretical calculations can be used to predict the molecular weight of a protein based on its amino acid sequence and known molecular weights of individual amino acids. 中文回答: his-sumo分子的分子量约为27 kDa(千道尔顿)。his-sumo融合蛋白由聚组氨酸标签(His标签)融合到小的泛素样修饰剂(SUMO)组成...
SUMO tag in recombinant SUMO fusion proteins. The CoolCutter™ enzyme recognizes the tertiary structure of SUMO rather than an amino acid sequence for a clean release of the desired peptides. A high activity in a wide range of pH, salt and detergent concentrations makes CoolCutter™ SUMO ...
amino acid sequenceSignificantly increased stability with SUMO fusion can be used for small peptide productionT7lac promoter for high level protein expressionN terminal 6xHis tag for protein detection and purification pETsumo 载体序列载体序列 CAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAA...
It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO protease itself has a His tag for easy removal from the protein mix after cleavage.Find additional tips, troubleshooting help, and resources within our ...
SUMO protease / ULP1 is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. Catalog No. YXX51101 Form Liquid Storage buffer Supplied as solution form in PBS pH 7.5. ...
(D) Alignment of amino acid sequences of SUMO5, SUMO1, SUMO2, SUMO3 and SUMO4. Conserved SUMO conjugation motifs are boxed by solid lines. The di-glycine motif whose removal is required for SUMO activation is boxed by dashed lines. *indicates amino acid residues in SUMO5 that were ...
But, in contrast to ubiquitylation, sumoylation does not tag proteins for degradation, but seems to enhance their stability or modulate their subcellular compartmentalization.关键词: Animals Humans Saccharomyces cerevisiae Ligases Ubiquitin-Conjugating Enzymes Proteins Sequence Homology, Amino Acid Amino Acid...