Because both strands have the same sequence running in opposite directions the enzymes make double-stranded breaks, which, if the site of cleavage is off-centre, generates fragments with short single-stranded t
The gene for Ulp1_WT was cloned into vector pET28b (Novagen) with an N-terminal hexahistidine tag followed by a thrombin cleavage site. Ulp1_R1–4 were cloned into vector pET29b with a hexahistidine tag at both the N and C termini; these are plasmids pCDB325–328. Cth SUMO protease ...
Use SUMO Protease to cleave SUMO, resulting in the production of native protein with no extra amino acids added between the cleavage site and the start of your protein. Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center. Prokaryotic mRNAs contain ...
The bond within a substrate that is subject to enzymatic cleavage. Nuclear pore complex (NPC). Large multiprotein complex that forms a channel in the nuclear envelope of an eukaryotic cell. The NPC joins the inner and outer nuclear membranes and allows transport of proteins to and from the nu...
To elucidate if LMP1 expression was sufficient to inhibit SUMO-protease activity, the cleavage of SUMO-AMC was analyzed in a set of paired cell lines42,43,44. No EBV (BL41 EBV negative cells) are B-cells that are not infected with EBV so they fail to express LMP1. EBV WT (BL41 EBV...
The Ulp1 protease used to cleave the fusion protein may be the Ulp1 protease or the active Ulp1 protease fragment, Ulp1 (403-621). The Ulp1 protease rapidly and specifically cleaves the fusion proteins of the invention at the Ulp1 cleavage site. The amino acid sequence recognized by a Ulp1 ...
all residual Cohesin is released at anaphase through cleavage of the Scc1p by a specific protease, called Separase [57]. Before anaphase, Separase is inhibited through interaction with Securin, a key APC/C target, whose degradation allows Separase activity. The Pds5p protein associates with Cohesi...
First, the SUMO molecule must be proteolytically processed by SUMO pepti- dases/isopeptidases to cleave-off a short C-terminal sequence, thus exposing an internal di-Gly sequence that becomes the carboxyl end of the mature SUMO protein (i.e., the proteolytically processed form). Second, SUMO...
2000. Based on the close homology to the protease domains of this family, the murine clone was named SUMO-1 Protease-1, SuPr-1. Download: Download high-res image (1MB) Download: Download full-size image Figure 1. SuPr-1 Identification and Transcriptional Activity (A) Amino acid sequence ...
The following search criteria were used: taxonomy, Homo sapiens; mass error tolerance for parent ion and fragment ions, 0.3 and 0.3 Da, respectively; fixed modification, cysteine carbamidomethylation; variable modification, methionine oxidation; enzyme, trypsin; and number of missed cleavages, one. ...