SUMO 蛋白酶(也称为 Ulp)是酿酒酵母中 ULP1(Ubl 特异性蛋白酶 1)的重组片段。其对 SUMO 蛋白融合具有高度特异性,识别 SUMO 的三级结构而不是氨基酸序列。SUMO 蛋白酶特点: •高度活性切割 •无非特异性蛋白水解(高度特异性剪切*) •在 2°C 至 37°C 下具有活性 ...
It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO Protease features:• Highly active cleavage• No non-specific proteolysis (highly specific cleavage*)• Activity from 2°C to 37°C• A six-histidine...
It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO Protease features: Highly active cleavage No non-specific proteolysis (highly specific cleavage*) Activity from 2°C to 37°C A six-histidine sequence to ...
Varying concentrations of EGFP-Smt3GGGG-mCherry were preincubated in 96-well half-area plates (Corning, catalog no. 3686) for 10 min at 30 °C, and SUMO protease was added to initiate the reaction (0.1–2 nm enzyme). The time course of the cleavage reaction was monitored by loss of ...
Because both strands have the same sequence running in opposite directions the enzymes make double-stranded breaks, which, if the site of cleavage is off-centre, generates fragments with short single-stranded tails; these can hybridize to the tails of other fragments and are called sticky ends. ...
(5.5-9.5), etc. SUMO protease also has a polyHis tag, which is convenient for Ni-NTA removal after cleavage of fusion protein. SUMO Protease is a recombinant protease expressed by affinity purification from E.coli. & & storage conditions: & & SUMO protease -80 ℃ for long-term storage,...
Cellular biology Structure-function analysis of the yeast SUMO protease Ulp2 YALE UNIVERSITY Mark Hochstrasser KroetzMary BernadetteIn the yeast , two SUMO proteases, Ulp1 and Ulp2, are responsible for the cleavage of SUMO from a substrate protein, termed desumoylation. These two enzymes are ...
SUMO protease can be used to cleave SUMO tags from recombinant fusion proteins. The enzyme is active over extensive temperature ranges from 4°C to 30°C and pH ranges from 7.0 to 9.0. The optimal temperature for cleavage is 30°C and SUMO Protease is easy for removal from the cleavage ...
2.0 版本 说明书 产品名称:SUMO 蛋白酶 货号:SP-100、SP-200、SP-300、SP-401 描述: SUMO Protease也称称 Ulp)是一种具有非常高活性的半胱氨酰蛋白酶。它是源自酿酒酵母的 Ulp1也Ubl 特异性蛋白酶 1)的重组片段,并通过使用多组氨酸标签的亲和层析从大肠杆菌中纯化。 SUMO 蛋白酶可识别泛素样 (UBL) 蛋白小...
classes of SUMO proteases. SUMO proteases are now known to fall into one of three distinct classes: the well-characterized UBL-specific protease (Ulp) and sentrin-specific protease (SENP) class; the desumoylating isopeptidase (DESI) class; or the ubiquitin-specific protease-like 1 (USPL1) ...