Chromosome structure at the multi-nucleosomal level has remained ambiguous in spite of its central role in epigenetic regulation and genome dynamics. Recent investigations of chromatin architecture portray diverse modes of interaction within and between nucleosome chains, but how this is realized at the ...
Cryo-electron microscopy reveals high-resolution structures of 30-nm chromatin fibers in vitromarine-derivedBacillus marinuselectroporationmacrolactingreen fluorescent proteinphosphopantetheinyl transferaseHuman chromosomes are the largest and most visible structures involved in the transfer of genetic information.The...
Early electron microscopy (EM) studies of native chromatin fibers have led to the proposal of two models for the 30-nm fiber: (i) the one-start Inter-fiber nucleosome–nucleosome interactions As summarized in Figure 2, Mg2+-dependent intra-fiber compaction and inter-fiber oligomerization of ...
PRC2 activity is regulated in part by the selective incorporation of one of two paralogs of the catalytic subunit, EZH1 or EZH2. Each of these enzymes has specialized biological functions that may be partially explained by differences in
cell domains exist in distinct cell lines. Perturbations of major epigenetic components and transcription mostly do not affect the frequency or strength of the single-cell domains. Increased chromatin compaction of inactive X chromosomes occurs at a length scale above that of the single-cell domains....
Lens epithelial cells are responsible for maintaining much of the homeostasis of the underlying fibers. Although the amount of UV radiation reaching the retina in the adult eye is very low (1% UV below 340nm and 2% between 340–360nm), much enough to degenerate outer nuclear layer area of ...
This review focuses on the origins, structures and functional aspects that are paradoxically less well described in the literature while numerous reviews are directed to the clinical application of cirDNA. Differentiation of the various structures and better knowledge of the fate of cirDNA would ...
Cryo-electron microscopy reveals high-resolution structures of 30-nm chromatin fibers in vitro 纤维染色电子显微镜高分辨率结构染色质人类染色体体外低温Human chromosomes are the largest and most visible structures involved in the transfer of genetic information.The most striking property of a chromosome is ...
For this reason, imaging/mapping nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin structure on genome function. An example is represented by the study of essential nuclear functions such as DNA transcription and replication, both occurring in the ...
Following central nervous system (CNS) injury, activated astrocytes form glial scars, which inhibit axonal regeneration, leading to long-term functional deficits. Engineered nanoscale scaffolds guide cell growth and enhance regeneration within models of