Chromatin structure plays a key role in regulating gene expression by allowing DNA accessibility to transcriptional machinery and transcription factors. The packaging of DNA into nucleosomes forms a closed structure that is not highly accessible to transcriptional elements whereas the open nucleosome ...
The state-of-the-art methods collected here are designed to analyze the relationship between chromatin structure and function, and to elucidate the molecular mechanisms that control such vital cellular functions as transcription, replication, recombination, and DNA repair. These proven methods cover a ...
Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length. Chromosome Res. 14, 17–25 (2006). CAS PubMed Google Scholar Fyodorov, D. V., Zhou, B. R., Skoultchi, A. I. & Bai, Y. Emerging roles of linker histones in regulating ...
W. Synthesis of ADP-ribosylated histones reveals site-specific impacts on chromatin structure and function. J. Am. Chem. Soc. 143, 10847–10852 (2021). Article CAS PubMed Google Scholar de Murcia, G. et al. Modulation of chromatin superstructure induced by poly(ADP-ribose) synthesis and ...
The complex life cycle of the malaria parasite Plasmodium falciparum involves several major differentiation stages, each requiring strict control of gene expression. Fundamental changes in chromatin structure and epigenetic modifications during life cycl
Natsume, R. et al. Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4.Nature446, 338–341 (2007).References 22 and 23 provide the first co-crystal structure of a histone chaperone in complex with its histone cargo, providing a molecular description of...
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Implicit in this analysis is the assumption that a cell's chromatin signature, or its epigenome, will be highly diagnostic of function. One metric of chromatin structure is accessibility of the DNA in bulk chromatin. DNA-modifying enzymes such as nucleases [1, 2] and methyltransferases [3–5]...
A greater understanding of genome structure and function has created new opportunities for intervening with biology and disease states by targeting cellular DNA or the associated proteins with small molecules. It is essential to validate target engagement for molecular probes and therapeutic drugs. ...
Besides chromatin modifications, chromatin-associated proteins such as TFs or remodeling proteins are important to regulate chromatin structure and function. In principle, our method is easily generalized to probe the nanoenvironments around the target sites of these proteins, such as spatial organization...