transcription. In celebration of Robert Feulgen’s ground-breaking discovery that allowed us to start seeing the genome, we discuss current models of chromatin structure and future challenges ahead.
chromatin reconstitutionin vitro analysislinker histoneDrosophilaA rigorous biochemical analysis of chromatin structure and function requires the assembly of ... E Bonte,PB Becker - 《Methods in Molecular Biology》 被引量: 117发表: 1999年 The role of histone variability in chromatin stability and foldi...
Structural insights into assembly and function of the RSC chromatin remodeling complex Article 07 December 2020 Cryo-EM structure of SWI/SNF complex bound to a nucleosome Article 11 March 2020 Structure of nucleosome-bound human PBAF complex Article Open access 10 December 2022 Data...
A greater understanding of genome structure and function has created new opportunities for intervening with biology and disease states by targeting cellular DNA or the associated proteins with small molecules. It is essential to validate target engagement for molecular probes and therapeutic drugs. ...
Root development and function have central roles in plant adaptation to the environment. The modification of root traits has additionally been a major driver of crop performance since the green revolution; however, the molecular underpinnings and the regulatory programmes defining root development and res...
Implicit in this analysis is the assumption that a cell's chromatin signature, or its epigenome, will be highly diagnostic of function. One metric of chromatin structure is accessibility of the DNA in bulk chromatin. DNA-modifying enzymes such as nucleases [1, 2] and methyltransferases [3–5]...
The molecular basis for depot-specific differences in gluteofemoral (GF) compared with abdominal (A) subcutaneous adipocyte function is poorly understood. In the current report, we used a combination of Assay for Transposase-Accessible Chromatin followed by sequencing (ATAC-seq), RNA-seq, and ...
Structure-function evolution of the Transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms p pBackground/p pExamination of ancient gene families can provide an insight into how the evolution of gene structure can relate to function. Functional ho... IH Still...
embryos, chromatin structure undergoes a process of loss and rebuilding20. In mouse embryos, higher order chromatin architecture gradually matures during development which is transcription independent17,18. However, little is known about the reprogramming of 3D chromatin structure during SCNT embryo ...
Overall, the current method is a useful tool to assemble nucleosomes with various histone variants, and to functionally determine the remodeling activities related to chromatin structure and physiological functions in plants. Methods Gene amplification by PCR using cDNA library ...