Using this method, we prepared high-quality strand-specific RNA-seq libraries from RNA extracted from the human malaria parasite Plasmodium falciparum. The libraries are compatible with Illumina's sequencers Genome Analyzer and Hi-Seq. The method can however be easily adapted to other platforms....
对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: --rf Assumes a stranded library fr-firststrand. --fr Assumes a stranded library fr-secondstrand. kallisto: --fr-stranded runs kallisto in strand specific mode, only fragments where the first ...
RSeQC判断链特异性(strand-specific) 对于strand-specific的RNA-seq而言,我们必须得知道它是哪一种建库方式,才能进行后续的定量分析。 stringtie: 代码语言:javascript 代码运行次数: --rf Assumes a stranded library fr-firststrand.--fr Assumes a stranded library fr kallisto: 代码语言:javascript 代码运行次数:0...
High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract ...
Despite the recent precipitous decline in the cost of genome sequencing, library preparation for RNA-seq is still laborious and expensive for applications such as high throughput screening. Limited availability of RNA generated by some experimental workf
Then, we applied strand-specific RNA-seq to investigate the effects of this microbiota on the global gene expression of EHEC O2621765 and O157EDL933 strains by comparison with their behavior in beef meat without microbiota. In strain O2621765, the expression of genes connected with nitrate ...
平台 MGISEQ-2000 MGISEQ-200 DNBSEQ-T7 DNBSEQ-T10×4 应用 WGS transcriptome lncRNA strand-specific transcriptome smRNA WES WGS或组装 QC non-strand specific transcriptome WGBS Demo数据 建库试剂盒名称平台读长样本样本信息下载 MGIEasy Fast RNA 文库制备试剂套装 DNBSEQ-T7 PE150 UHRR 查看详细 MGIEasy...
Pellets were washed once in 50 mM Tris pH 8.0 then RNA was digested at 37 °C overnight with 200 μg of RNase A (diluted in 10 mM Tris pH 7.5, 10 mM MgCl2). Proteins were degraded at 50 °C for 30 minutes with 400 μg of Proteinase K (diluted in 10 mM Tris pH 8.0). ...
MGIEasy Fast RNA 文库制备试剂套装 MGISEQ-2000 PE150 UHRR 查看详细 MGIEasy Fast RNA 文库制备试剂套装 DNBSEQ-T7 PE150 UHRR 查看详细 MGIEasy Fast RNA 文库制备试剂套装 DNBSEQ-T7 PE150 UHRR 查看详细 MGIEasy rRNA去除试剂盒V1.3 MGISEQ-2000 PE150 UHRR 查看详细 MGIEasy RNA方向性文库制备试剂套...
The amplified RNA molecules are used to prepare scMspJI-seq libraries and sequenced on an Illumina platform. Full size image To validate the method, we first applied scMspJI-seq to single E14TG2a (E14) mouse embryonic stem cells (mES) cells. As reported previously, we found that MspJI cuts...