star的twopassMode问题 如果只有一个样本,那么通常推荐加上这个--twopassModeBasic参数,可以保证更精准的比对情况,也方便去找变异,其官方文档解释如下: Annotated junctions will be included in both the 1st and 2nd passes. To run STAR 2-pass mapping for each sample separately,use --twopassMode Basic opt...
AI代码解释 ## 进行 reads 比对STAR--twopassMode Basic \--quantMode TranscriptomeSAM GeneCounts \--runThreadN6\--genomeDir index_dir \--alignIntronMin20\--alignIntronMax50000\--outSAMtypeBAMSortedByCoordinate \--sjdbOverhang149\--outSAMattrRGlineID:sampleSM:samplePL:ILLUMINA\--outFilterMismatch...
STAR --twopassMode Basic --quantMode TranscriptomeSAM GeneCounts --runThreadN 6 --genomeDir index_dir --alignIntronMin 20 --alignIntronMax 50000 --outSAMtype BAM SortedByCoordinate --sjdbOverhang 149 --outSAMattrRGline ID:sample SM:sample PL:ILLUMINA --outFilterMismatchNmax 2 --outSJfilter...
这种做法综合了多个样本的intron信息,比对的灵敏度会更高,缺点是操作比较繁琐。 per-sample 2-pass 对于单个样本,在比对时直接添加--twopassMode Basic参数,软件会自动进行两次比对,将第一次比对的SJ.out.tab加入到索引,然后重新比对。这种方法操作简单,适用于单个样本的2-pass 比对。 更多参数和用法请参考官方文档...
的2-pass mapping,可以直接用--twopassMode Basic参数将第两步mapping中的make index省去,直接再mapping STAR --runThreadN 20 --genomeDir ~/reference/index/STAR/mm10/ \ --twopassMode Basic \ --readFilesIn SRR3589959_1.fastq SRR3589959_2.fastq \ --outSAMtype BAM SortedByCoordinate \ --...
If your boarding pass does not carry the Star Alliance Gold indicator, you will need to also show your valid Star Alliance Gold card. The lounge displays the Star Alliance Gold logo at the entrance. You are entitled to bring one eligible guest travelling on the same Star Alliance flight depa...
variable needs to hold whatever text was passed into the function call since we want to operate on strings. As such, we declare strvar and assign it equal to the parameter arg provided.strvar := argThen, after declaring our second variable, we initialize it similarly but instead pass in ...
With only a single pass, the logo was approved by Rick Berman. ProductionIn the spring of 1996, newly-recruited director Jonathan Frakes and producer Rick Berman cast their three "guest stars". Two-time Academy Award winner Tom Hanks, an admitted Trekker, was slated to play Zefram Cochrane ...
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And we are! We arrive at 7 p.m. and head to our two homes. The girls, Adam, and I are staying in one house, and the crew is staying down the road. I go to get a spray tan (I need to look good for the Expo; fans expect to see me at my best!). Then I rest before th...