The present invention discloses a Thermus single-stranded DNA TC16 binding proteins and polynucleotides encoding this protein, the protein derived from Thermus ThermusTC16 strain, has the amino acid sequence shown SEQIDNO.1; the protein in the 20- 80 ℃ have the ability to single-stranded DNA...
Mapelli M, Panjikar S, Tucker PA (2005) The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding. J Biol Chem 280: 2990-2997.Mapelli M, Panjikar S, Tucker PA. 2005. The crystal structure ...
Given the prominent role of RPA as a ssDNA-binding protein, we went on to test whether ssDNA affects the phase-separation behavior of RPA. Strikingly, ssDNA, but not sequence-matched annealed double-stranded DNA (dsDNA), greatly enhanced RPA phase separation (Fig. 2d and Extended Data Fig. ...
The methods particularly involve the detection of overexpression of an ssDNA binding protein (SSB) or polypeptide comprising the following amino acid sequence: FX1X2DX3KPGLKNLNX4X5FIVLEX6GRVTKTKDGHEVRX7CKVADKTGSIX8ISVWDX9X10GX11LIQPGDI IRLTX12GYASX13X14KGCLTLYTGRGGX15LQKIGEFCMVYSEVPNFSEPNP...
boundproteinmustoccurfrequentlyin thecell,butlittleisknownaboutitsoutcome.Here wedevelopedamulticolorsingle-moleculefluores- cenceapproachtosimultaneouslymonitorsingle- strandedDNA(ssDNA)translocationbyahelicase andthefateofanotherproteinboundtothesame DNA.Distance-dependentfluorescencequenching bytheiron-sulfur...
We have isolated dosage-dependent suppressor genes (designatedHMD genes) of the mating defect caused byCLN3-2, a dominant mutation inCLN3,HMD2 andHMD3 are identical toSTE4 andSTE5, respectively,HMD1 is an essential gene that encodes a protein containing a putative RNA binding domain. ...
Consequently, considerably fewer computational studies have explored interactions between proteins and single-stranded nucleic acids compared with protein interactions with double-stranded nucleic acids. Here, we report a newly developed energy-based coarse-grained model to predict the structure of ssDNA-ssD...
similar to HIV [61], also induces them to express the inhibitory receptor Fc receptor-like protein (FcRL)4 [54,60], whereas circulating CD21– B cells in healthy individuals [63,64], or those afflicted by the autoimmune disease systemic lupus erythematosus (SLE) [63], lack FcRL4 expressi...
Displacement of ssDNA-binding protein (SSB) from single-stranded DNA (ssDNA) is necessary for filament formation of RecA on ssDNA to initiate homologous recombination. The interaction between RecO and SSB is considered to be important fo... I Jin,T Nagae,M Mishima,... - 《Journal of Biologic...
However, the site size of the 8 kDa domain-ssDNA complex is ten nucleotides, suggesting that the formation of the (pol β) 5 mode is accompanied by a significant conformational transition of the intact protein. A higher intrinsic affinity, a higher net number of ions released, and a lower ...