Escherichia colisingle-stranded DNA (ssDNA) binding protein (SSB) is the defining bacterial member of ssDNA binding proteins essential for DNA maintenance. SSB binds ssDNA with a variable footprint of ∼30–70 nucleotides, reflecting partial or full wrapping of ssDNA around a tetramer of SSB. W...
Finally, I challenged the above assumption by showing that the PAAD domain from the HIN-200 family member, IFI16, has all the characteristics of a single stranded nucleic acid binding protein, motivating further studies for the discovery of new PAAD-ligand in...
The binding mechanism suggests that coarse-grained models are appropriate to study the motion of SSBs along ssDNA, which is expected to be central to the function carried out by the SSBs. 展开 关键词: ssDNA protein-DNA interaction coarse-grained model ...
The dynamic binding of RPA to ssDNA has been proposed to be crucial for its biological functions3,13. While RPA offers high affinity and protection of ssDNA, the shifting of RPA ssDNA binding modes likely frees ssDNA for downstream protein factors to grab ssDNA and act upon. In vitro, RPA ...
The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA The Escherichia coli RecF, RecO and RecR pro teins have previously been implicated in bacterial recombinational DNA repair at DNA gaps. The RecOR-facilitat... J.,M.,Bork - 《Embo Journal》 被引量: 195发...
Given the prominent role of RPA as a ssDNA-binding protein, we went on to test whether ssDNA affects the phase-separation behavior of RPA. Strikingly, ssDNA, but not sequence-matched annealed double-stranded DNA (dsDNA), greatly enhanced RPA phase separation (Fig. 2d and Extended Data Fig. ...
Although these latter data indicate that atypMBCs might directly contribute to the humoral anti-malarial immune response by participating in antibody secretion, other evidence suggests a more innate function of these cells; in addition to increasing the proportion of atypMBCs, malaria exposure, ...
The function of the ATR (ataxia-telangiectasia mutated- and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex is crucial for the cellular response to replication stress and DNA damage. Here, we show that replication protein A (RPA), a protein complex that associates with sing...
The Ff gene 5 protein (g5p) is considered to be a nonspecific single-stranded DNA binding protein, because it binds cooperatively to and saturates the Ff bacteriophage single-stranded DNA genome and other single-stranded polynucleotides. However, the binding affinity Kω (the intrinsic binding cons...
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