E.coli Single-Stranded DNA Binding Protein与ssDNA的结合可稳定与保护单链DNA免被DNase降解或形成二级结构,同时也可使DNA双螺旋不稳定,促使DNA聚合酶与底物结合,进而提高DNA聚合酶的催化活性,改善PCR扩增效率。 因此E.coli Single-Stranded DNA Binding Protein在PCR扩增、DNA测序等方面具有广泛应用。 产品效果 ...
Single-strand deoxyribonucleic acid (ssDNA) binding protein derived from thermus TC16 and polynucleotide for coding protein本发明公开了一种栖热菌TC16的单链DNA结合蛋白和编码此蛋白的多核苷酸,该蛋白来源于栖热菌ThermusTC16菌株,具有SEQIDNO.1所示的氨基酸序列;该蛋白在20-80℃均具有与单链DNA结合蛋白结合...
2020年10月匈牙利罗兰大学Gábor M. Harami等研究人员在《PNAS》(综合性期刊1区,IF=11.205)发表了题为“Phase separation by ssDNA binding protein controlled via protein?protein and protein?DNA interactions”的文章。本文报道了一种细胞如何动员DNA修复蛋白及时响应基因组应激并启动DNA修复单链DNA的分子机制。作者...
Miao Y*, Jiang J, Ren Y and Zhao Z,2013, ssDNA binding protein WHIRLY1 repressWRKY53expression and senescence in develpomental dependent manner in Arabidopsis. Plant Physiol163(2):745-756. Ren Y, Li Y, Jiang Y, W...
The methods particularly involve the detection of overexpression of an ssDNA binding protein (SSB) or polypeptide comprising the following amino acid sequence: FX1X2DX3KPGLKNLNX4X5FIVLEX6GRVTKTKDGHEVRX7CKVADKTGSIX8ISVWDX9X10GX11LIQPGDI IRLTX12GYASX13X14KGCLTLYTGRGGX15LQKIGEFCMVYSEVPNFSEPNP...
The eukaryotic single-stranded DNA (ssDNA)-binding protein Replication Protein A (RPA) plays a crucial role in various DNA metabolic pathways, including DNA replication and repair, by dynamically associating with ssDNA. While the binding of a single RPA molecule to ssDNA has been thoroughly studied...
Given the prominent role of RPA as a ssDNA-binding protein, we went on to test whether ssDNA affects the phase-separation behavior of RPA. Strikingly, ssDNA, but not sequence-matched annealed double-stranded DNA (dsDNA), greatly enhanced RPA phase separation (Fig. 2d and Extended Data Fig. ...
intensity, usually 10%–50%, upon binding of a protein in close proximity (Fischer et al., 2004; Luo et al., 2007; Myong et al., 2009). This effect is markedly different from the almost 100% Cy3 quenching observed in our study (Figure 1B and Figure S1). ...
Mapelli M, Panjikar S, Tucker PA (2005) The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding. J Biol Chem 280: 2990-2997.Mapelli M, Panjikar S, Tucker PA. 2005. The crystal structure ...
The single-stranded DNA (ssDNA)-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetr...