small RNA sequencingSmall RNA (sRNA) profiling of Extracellular Vesicles (EVs) by Next-Generation Sequencing (NGS) often delivers poor outcomes, independently of reagents, platforms or pipelines used, which contributes to poor reproducibility of studies. Here we analysed pre/post-sequencing quality ...
ArnA和6C RNA是目前在谷氨酸棒杆菌中通过实验确定的2条sRNA，ArnA位于CGL_RS11480的上游反义链上，6C RNA则位于CGL_RS01570上游的正义链上，它们不需要伴侣蛋白，可直接同靶基因mRNA相互作用。本研究通过对不同生长时期的谷氨酸棒杆菌进行深度测序，结合多个RNA sequencing (RNA-Seq)实验建立测序文库，通过基于RNA-S...
3. DNA sequencing: The amplified 16S rRNA gene is then subjected to DNA sequencing. There are several sequencing technologies available, such as Sanger sequencing or next-generation sequencing (NGS) platforms. The sequencing results in obtaining the nucleotide sequence of the 16S rRNA gene. 4. Seq...
原文:Deep sequencing-based identification of small regulatory RNAs in Synechocystis sp. PCC 6803 期刊:PLoS One本文通过细菌小RNA测序在细菌Synechocystis sp. PCC 6803中鉴定出5211个小RNA,并利用sRNA-PCR验证了其中的27个。通过进一步的sRNA靶基因预测发现:这些sRNA广泛参与糖酵解、三羧酸循环、脂肪酸代谢等...
Han X, Yin H, Song X, et al. Integration of smallRNAs, degradome and transcriptome sequencing in hyperaccumulatorSedum alfrediiuncovers a complex regulatory network and provides insights into cadmium phytoremediation:[J]. Plant Biotechnology Journal, 2016, 14(6):1470-1483....
Comparative pathogen genomics and deep sRNA sequencing based on microbiome projects would promote exploration of key trans-kingdom sRNAs involved in plant—F. oxysporum interactions, providing new efficient targets for crop protection tools such as SIGS....
With the development of high-density tiling arrays and RNA deep sequencing, studies of non-coding small RNAs attracts more and more attention, and meanwhile it is evident that non-coding small RNAs play a crucial role in many biological processes such as environmental sensing and stress adaptation...
IGV-sRNA It is a modified version of IGV, which is focused on small RNA sequencing data analysis. Most modification are based on the demand of our data analysis in XiaLab. Motivation I have tried twice to design and implement a genome browser, and I falied. In both tries, I have found...
Sequencing is the key method to study the impact of short RNAs, which include micro RNAs, tRNA-derived RNAs, and piwi-interacting RNA, among others. The first step to make use of these reads is to map them to a genome. Existing mapping tools have been developed for long RNAs in mind,...
Since the widespread applications of next-generation sequencing methods, the capability of sequencing technologies to generate millions of reads per sRNA-seq library has substantially enhanced the discovery of sRNAs (Axtell and Meyers, 2018). Given the unique characteristics of different types of sRNAs,...