5%脱脂奶粉室温封闭 2 h,分别加入抗 Flag 小鼠单克隆抗体 ( 购自美国 Sigma 公司 )、抗 C/EBPα 和 SREBP - 1 兔多克隆抗体 ( 购自美国 Abcam 公司 )、抗 FASN 和 ACC1 兔多克隆抗体 ( 购自美国 Proteintech 公司 )、...
Antibodies and reagents Antibodies used in the research were Anti-SCD1 from Cell Signaling Technology; Anti-FASN/β-actin/vimentin/N-cadherin and Anti-E-cadherin from Proteintech; Anti-SREBP-1 from BD Pharmingen. Palmitic Acid (16:0) (PA), Oleic acid (OA) were purchased from Solarbio life ...
and incubated overnight at 4 °C with the primary antibodies in the blocking buffer containing 0.2% Tween-20. The primary antibodies used were EXO1 (Cat: 16253-1-AP, Proteintech, China) and ACTB (Cat: AC004, Abclonal, China). For secondary antibody treatment, the membranes were either bl...
The membranes were incubated with anti-GK5 (Proteintech Group, IL, USA), anti-EGFR (Cell Signaling Technology (CST), MA, USA), anti-survivin (CST), anti-PLK1 (CST), anti-Bcl2 (CST), anti- cleaved caspase-3 (Asp175, CST), anti-caspase-3 (CST), anti- cleaved PARP (Asp214, CST...
SRSF1 (WB 1:1000) Proteintech Cat#12929-2-AP; RRID: AB_2187211 ACLY (WB 1:1000) Proteintech Cat#15421-1-AP; RRID: AB_2223741 FASN (WB 1:1000) Proteintech Cat#10624-2-AP; RRID: AB_2100801 SCD1 (WB 1:1000) Abcam Cat#ab19862; RRID: AB_445179 ACC1 (WB 1:1000) Proteintech ...
Western blot analysis for HMGCR (BS6625, Bioworld Technology, diluted 1:1000), SREBP2 (14508-1-AP, Proteintech, diluted 1:1000), CYP7A1 (AB79847, Abcam, UK, diluted 1:1000), CYP27A1 (BS2192, Bioworld Technology, diluted 1:500), BHMT (15965-1-AP, Proteintech, diluted 1:1000), ...
5%脱脂奶粉室温封闭 2 h,分别加入抗 Flag 小鼠单克隆抗体 ( 购自美国 Sigma 公司 )、抗 C/EBPα 和 SREBP - 1 兔多克隆抗体 ( 购自美国 Abcam 公司 )、抗 FASN 和 ACC1 兔多克隆抗体 ( 购自美国 Proteintech 公司 )...
5%脱脂奶粉室温封闭2 h,分别加入抗Flag小鼠单克隆抗体(购自美国Sigma公司)、抗C/EBPα和SREBP-1兔多克隆抗体(购自美国Abcam公司)、抗FASN和ACC1兔多克隆抗体(购自美国Proteintech公司)、抗β-肌动蛋白(β-actin)兔多克隆抗体(购自北京锐抗生物科技有限公司),4℃孵育过夜;次日用PBST洗膜,加入辣根过氧化物酶标记...