他们的最新研究“CXCL9:SPP1 macrophage polarity identifies a network of cellular programs that control human cancers”已于著名期刊《Science》上发表。作者发现,由 CXCL9 和 SPP1 这两个基因的表达所定义的巨噬细胞极性的变化是肿瘤微环境的一个简单而关键的特征。CXCL9:SPP1的比例可以描述癌症中抗肿瘤免疫细...
CANCER IMMUNOLOGYCXCL9:SPP1 macrophage polarity identifies a networkof cellular programs that control human cancersRuben Bill 1,2,3,4,5 †, Pratyaksha Wirapati 1,2,3 †, Marius Messemaker 4,6 , Whijae Roh 7 , Beatrice Zitti 1,2,3 ,Florent Duval 1,2,3 , Máté Kiss 1,2,3 , ...
2024年2月,广西医科大学附属肿瘤医院的向邦德教授团队发表一篇题为“Integrating single‑cell and bulk RNA sequencing reveals CK19 + cancer stem cells and their specific SPP1 + tumor‑associated macrophage niche in HBV‑related hepatocellular carcinoma”的研究成果。本研究通过整合单细胞和Bulk RNA测序数...
c: SPP1+ macrophage在Tumor中的比例显著升高; d: TCGA-COAD数据集中,SPP1+ macrophage在tumor中的比例也显著高; e: 高浸润SPP1+ macrophage的病人,预后差; f: 用于鉴定9个细胞亚型的marker的小提琴图; g: 用流式分选SPP1+ macrophage,验证在tumor中显著高; ...
In conclusion, we propose anSPP1+macrophage model in CRC, highlighting such macrophages as a promising therapeutic target due to their malignancy markers. 展开 关键词: KeywordsColorectal cancerImmunotherapySingle-cell RNA sequencingSpatial transcriptomicsSPP1+macrophages DOI: 10.1016/j.gendis.2024.101340...
g: 用流式分选SPP1+ macrophage,验证在tumor中显著高; h: RNA velocity分析表明,SPP1+ macrophage有可能起源于THBS1+ macrophage; i/j/k/l: 用pySCENIC分析,10个细胞亚型的top 转录因子和调节子,发现STAT1 是SPP1+ macrophage特异性表达; FAP+ fibroblasts 和 SPP1+ macrophage相关性分析 ...
[30] Arai S, Kitada K, Yamazaki T, et al. Apoptosis inhibitor of macrophage protein enhances intraluminal debris clearance and ameliorates acute kidney injury in mice[J]. Nat Med, 2016, 22(2): 183-93. [31] Moeckel GW. Pathologic perspectives on acute tubular injury assessment in the ...
THP-1 cells co-cultured with A549 cells attenuated CD4 + T-cell activation, whereas SPP1 inhibition restored T-cell activation. These results highlight the importance of SPP1 in mediating macrophage polarization and lung cancer evasion, suggesting a potential therapeutic target for lung cancer. ...
Moreover, we find that platelets, the most abundant source of CXCL4 in vivo, drive profibrotic Spp1 macrophage differentiation. Single nuclear RNA sequencing with ligand-receptor interaction analysis reveals that macrophages orchestrate fibroblast activation via Spp1, Fn1, and Sema3 crosstalk. ...
We also discovered the heterogeneity within TAMs, among which a group of suppressive TAMs named TAM-SPP1 demonstrated a significant association with Epidermal Growth Factor Receptor ( EGFR ) amplification, impaired T cell response and unfavourable patient survival outcomes. Furthermore, by leveraging ...