While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border regions and combining single-cell RNA-sequencing with high-dimensional cytome
(3)培养:将上清液轻轻弃去,加2 mL完全培养基,轻柔悬起细胞,然后将所有细胞悬液移至含有3 mL完全培养基的T25培养瓶中,于37℃,5% 二氧化碳培养箱中培养; (4)观察:每日观察细胞生长状态(培养基颜色、形态与密度等)并拍照。 传代 细胞密度达80%-90%开始传代 (1) 清洗:无菌下吸出培养基,用37℃预热的PBS清洗...
To infer the aging process for the microglia/macrophage clusters (n = 1,121 nuclei) generated in Seurat, we applied Monocle3 (refs. 44,70). Monocle3 uses dimensionality reduction to place single cells in a two-dimensional space, removes batch effects by mutual nearest neighbor alignment ...
Single cell spatial transcriptomic characterization using the CosMx Spatial Molecular Imager platform was conducted to analyze HIV-infected cells in formalin-fixed paraffin-embedded sections of splenic tissue surgically obtained from an HIV-infected humanized mouse model. Regulation of over a thousand human...
英文名称:Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (non-denatured)总访问:938 国产/进口:进口半年访问:24 产地/品牌:Abnova产品类别:抗体 规格:200 μg最后更新:2024-12-5 货号: CAS 号: 参考报价:1500元 ...
- 《Cell & Tissue Research》 被引量: 191发表: 1988年 Macrophage-like cells in the muscularis externa of mouse small intestine (p 77-86) Macrophage-like cells in the muscularis externa of mouse small intestine, Anat. Rec. 213 , 77–86.Mikkelsen HB, Thuneberg L, Rumessen JJ, Thorball N...
Single-cell studies have revealed IPF-associated cell states, including atypical epithelial cells, fibroblasts4,5and profibrotic alveolar macrophages6,7. Interestingly, a novelKRT5−/KRT17+aberrant basaloid (AbBa) epithelial cell population has been independently identified in multiple studies4,5,8,9,...
Three-dimensional diffusion map embedding was used to identify a start cell (within the E-SLAM population) and end cells for each of the 3 lineages (E, erythroid; GM, granulocyte-macrophage; and L, lymphoid). Identifying broad branches between start and end cells was done by finding cells ...
and green cells: trunk (10.1%), endocrine progenitor (EP; 1.8%), Chg-expressing (Chg; 6.1%), α cell (α; 21.0%), two β cell (β1, 20%, and β2, 11.2%), δ cell (δ; 15.0%), Ghrl cell (7.7%), S-phase cell (S; 3.8%), stellate (2.7%), and macrophage (ϕ; 0.6...
a, b, Diversity score at different cluster resolutions for FACS brain myeloid microglia cell (a) and droplet kidney macrophage (b). n = 14 mice for a and n = 16 mice for b. All data are expressed as quantiles. The P values were obtained using a linear regression and two-sided F-tes...