原文链接:Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana 分裂荧光素酶互补成像分析研究本氏烟草中的蛋白质之间相互作用 Bio-protocol 简介 Bio-protocol于2011年在斯坦福大学创建, 致力于搭建全球权威的、高质量的生物实验方案分享平台,以助力科学发现。Bio-p...
By applying split-luciferase complementation to the interaction between the G伪q protein and its effector the phospholipase C-尾3 (PLC-尾3), we introduce a protocol with a conventional plate reader at high throughput. The method is applicable to live cells and additionally allows imaging of the...
The recently established split-luciferase complementation (Split-LUC) imaging assay has several advantages compared to other approaches to detect PPIs in planta: it is a relatively simple and fast method to detect PPIs in vivo; the results are quantitative, with high sensitivity and low background;...
Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, Mendel RR, Hansch R: Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. The Plant journal 2011, 67(3):542–553. doi:10.1...
Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, Mendel RR, Hänsch R (2011) Quantitative analysis of dynamic protein–protein interactions in planta by a floated‐leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. Plant J 67:542–553 Article CAS Google ...
In this study we have adapted a split-luciferase complementation assay (LCA) for detecting the assembly of the voltage-gated Na+ (Nav) channel C-tail ... A Shavkunov,N Panova,A Prasai,... - 《Assay Drug Dev Technol》 被引量: 63发表: 2012年 A novel luminescent biosensor for rapid mo...
In conclusion, we have developed a novel monomolecular bioluminescent Ras signaling biosensor based on luciferase fragment complementation strategy. This biosensor allows non-invasive, real time, dynamic and reversible imaging as well as quantification of endogenous Ras activity in living cells and in vivo...
Recruitment of β-arrestin may also be measured using protein-protein interaction assays that utilize bioluminescence resonance energy transfer (BRET)18, fluorescence resonance energy transfer (FRET)19 or complementation strategies based on β-galactosidase20 or luciferase fragments21. Likewise, we have ...
Strategies to monitor protein-protein interactions (PPIs) in cells allow for biophysical characterization of the proteome in its native environment while providing convenient assays for developing inhibitors for PPIs. Split-protein complementation methods using luciferase, a class of oxidative enzymes that ...
[18]Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, et al. (2011) Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. Plant J 67: 542–543. ...