Hosseinkhani (2014) Split-luciferase complementary assay: Applications, recent developments, and future perspectives. Anal. Bioanal. Chem. 406, 5541-5560.Azad, T.; Tashakor, A.; Hosseinkhani, S. Split-luciferase complementary assay: Applications, recent developments, and future perspectives. Anal....
The split-luciferase complementation assay makes the study of two or more interacting proteins possible. In this technique, each of the two domains of luciferase is attached to each partner of two interacting proteins. On interaction of those proteins, luciferase fragments are placed close to each ...
REVIEWSplit-luciferase complementary assay: applications,recent developments, and future perspectivesTaha Azad & Amin Tashakor & Saman HosseinkhaniReceived: 26 February 2014 /Revised: 22 May 2014 /Accepted: 16 June 2014 /Published online: 8 July 2014# Springer-Verlag Berlin Heidelberg 2014Ab...
Split-luciferase complementary assay: applications, recent developments, and future perspectives Bioluminescent systems are considered as potent reporter systems for bioanalysis since they have specific characteristics, such as relatively high quantum ... Taha,Azad,Amin,... - 《Analytical & Bioanalytical Ch...
In the case of co-transfection with siRNA and complementary DNA plasmids, plasmid transfection was performed at 24 h after siRNA transfection, and cells were then cultured for an additional 48 h. The knockdown efficiency was confirmed by immunoblotting. For evaluation of protein stability in the ...
Assessment of the interactions between a drug and its protein target in a physiologically relevant cellular environment constitutes a major challenge in the pre-clinical drug discovery space. The Cellular Thermal Shift Assay (CETSA) enables such an asses
Split-luciferase complementary assay of NLRP3 PYD-PYD interaction indicates inflammasome formation during inflammation - ScienceDirectMohsen Isazadeh aMojdeh Amandadi aFarnaz Haghdoust aShima Lotfollazadeh bMar Orzáez cSaman Hosseinkhani a
Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule... AR Noori,A Tashakor,M Nikkhah,... - 《Biochimie》 被引量: 0发表: 2020年 加载更多来源...
A novel luminescent biosensor for rapid monitoring of IP3 by split- luciferase complementary assay. Biosens Bioelectron. 2013; 41:642-648.Ataei, F., M. Torkzadeh-Mahani and S. Hosseinkhani (2012) A novel luminescent biosensor for rapid monitoring of IP3 by split- luciferase complementary assay...
caspase-3 and Linker-BIR2 were used for designing two reporters based on split luciferase complementary assay to develop a luminescent probe.Caspase3-CLuc and BIR2-NLuc fragments were ligated in pET-28a(+),overexpressed in E.coli BL21(DE3)and purified to homogeneity.Interaction between caspase-3...