BCA protein assay for glycerol assays Reagents 热度: activate capture and digital counting (ac dc) assay for protein biomarker detection integrated with a self-powered microfluidic cartridge.[2019][ 热度: FireflyLuciferaseComplementationImagingAssayforProtein-ProteinInteractionsin ...
Luciferase Complementation Assay (LCA) Service Overview The Luciferase Complementation Assay (LCA) is an advanced, highly sensitive method designed for studying protein-protein interactions. In this assay, luciferase is split into two fragments: the N-terminal fragment (NLuc) and the C-terminal fragm...
Luciferases have served a number of purposes in biomedical applications, including within reporter gene and split reporter complementation assays. These proteins, however, have not evolved for the purpose of biomedical research, and it is not surprising that the utility and robustness of these assays...
Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gat... SummaryDynamic protein–protein interactions are essential in all cellular and developmental processes. Protein-fragment complementation assays allow ...
1. Luciferase complementation assays and their use for the network analysis of protein–protein interactions in animal cells 2. Use of luciferase complementation assays for the network analysis of protein–protein interactions in plant cells 3. Six types of luciferase with different enzymatic characterist...
Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, Mendel RR, Hansch R: Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. The Plant journal 2011, 67(3):542–553. doi:10.1...
Luciferase protein complementation assays for bioluminescence imaging of cells and mice. Protein fragment complementation assays (PCAs) with luciferase reporters currently are the preferred method for detecting and quantifying protein-protein i... GD Luker,KE Luker - 《Methods in Molecular Biology》 被引...
Misawa N, Kafi A, Hattori M, Miura K, Masuda K, Ozawa T (2010) Rapid and high-sensitivity cell-based assays of protein−protein interactions using split click beetle luciferase complementation: an approach to the study of G-protein-coupled receptors. Anal Chem 82:2552–2560 Article CAS Go...
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[18]Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, et al. (2011) Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. Plant J 67: 542–543. ...