原文链接:Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana 分裂荧光素酶互补成像分析研究本氏烟草中的蛋白质之间相互作用 Bio-protocol 简介 Bio-protocol于2011年在斯坦福大学创建, 致力于搭建全球权威的、高质量的生物实验方案分享平台,以助力科学发现。Bio-p...
Split-luciferase complementation assayProductive viral infection entails highly regulated and sequential protein-protein interactions between viral factors and between virus and host factors. Deciphering such interactions is of paramount importance for a better understanding of virus infection cycles and the ...
The split-luciferase complementation assay makes the study of two or more interacting proteins possible. In this technique, each of the two domains of luciferase is attached to each partner of two interacting proteins. On interaction of those proteins, luciferase fragments are placed close to each ...
Splitluciferasecomplementation;Regulatedprotein-proteininteractionsBackgroundInteractomeanalysiscollectsdataofprotein-proteinin-teractionsoccurringincells.Ausefulapplicationoftheanalysisistoidentifyfunctionalmodulesofsignaltrans-ductionpathwaysinwhichanetworkofprotein-proteininteractionsmediatethesignals(Barabasietal.2011;Pawson...
and show that luciferase-based biosensors are powerfultools that can be applied for diagnostic and therapeuticpurposes.Keywords Splitluciferase . Luminescentbiosensor . Protein–proteininteraction . ProteincomplementationassayIntroductionBioluminescence has been observed in many organisms, suchasbacteria, fungi,alg...
体内研究方法包括酵母双杂交系统（Y2H），双分子荧光互补技术（BiFC, Bimolecular fluorescence complementation assay），萤光素酶片段互补技术Split-LUC（Split luciferase complementation assay），以及荧光共振能量转移法（FRET）。体外研究方法（in vitro）包括蛋白质免疫共沉淀法（Co-IP），GST融合蛋白pull-down技术，串联...
A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Reni...
split luciferase complementation assayThe expense and time required for in vivo reproductive and developmental toxicity studies have driven the development of in vitro alternatives. Here, we used a new in vitro split luciferase‐based assay to screen a library of 177 toxicants for inhibitors of ...
Here, we developed an in vitro split luciferase complementation-based assay to speed up screening of PA–PB1 interaction inhibitors. By screening 10,000 compounds, we identified two PA–PB1 interaction inhibitors, R160792 and R151785, with potent and broad-spectrum antiviral activity against a ...
protein complementationsplit renilla luciferase complementation systemSummary This chapter contains sections titled: Introduction Protein Splicing-based Split Firefly Luciferase System Split Luciferase Works as a Probe for Protein Interaction Split Renilla Luciferase Complementation System Time Course of the ...