Nano luciferaseco‐immunoprecipitationallosteric changeDeciphering protein﹑rotein interactions (PPIs) is fundamental for understanding signal transduction pathways in plants. The split firefly luciferase (Fluc) complementation (SLC) assay has been widely used for analyzing PPIs. However, concern has risen ...
The split luciferase complementation (SLC) assay is one of methods that detect regulated protein-protein interactions in situ (Ozawa et al. [2001]; Kaihara et al. [2003]; Luker et al. [2004]). In the assay, N- and C-terminal fragments of luciferase are genetically fused to a protein ...
Deciphering protein-protein interactions(PPIs)is fundamental for understanding signal transduction pathways in plants.The split firefly luciferase(Fluc)complementation(SLC)assay has been widely used for analyzing PPIs.However,concern has risen about the bulky halves of Fluc interfering with the functions of...
split luciferase complementationProtein-protein interactions are important in human disease. Developing and refining tools to understand physical contacts between signaling proteins is crucial. This article describes a split luciferase complementation (SLC) method designed to discover inhibitors of protein-...
We report here a cell-based assay in which the formation of core dimers is indicated by split luciferase complementation (SLC). Making use of this model, 2 compounds, Arbidol (umifenovir) and 20-deoxyingenol, were identified from a library containing 672 compounds as core dimerization ...
Although both systems offer the potential to monitor recruitment of BioID with its target protein via complementation of the self-assembling proteins, we chose to pursue the GFP11 system because it enables easy monitoring of the expression and localization of the protein of interest in the cell ...