The split firefly luciferase complementation (SFLC) allows quantitative detection of in vivo protein-protein interaction in Arabidopsis mesophyll protoplasts.JianFeng LiJenifer BushYan XiongLei LiMatthew McCormack
each having advantages and disadvantages. Here we present split firefly luciferase complementation (SFLC) as a highly sensitive and noninvasive technique for in planta PPI investigation. In this assay, the separate halves of a firefly luciferase can come into close proximity and transiently restore its...