RESEARCHOpenAccessSplitluciferasecomplementationassaytodetectregulatedprotein-proteininteractionsinriceprotoplastsinalarge-scaleformatYukichiFujikawa1,TakahiroNakanishi1,HirokoKawakami1,KanakoYamasaki2,MasaHSato2,HiroyukiTsuji3,MakotoMatsuoka4andNaohiroKato5*AbstractBackground:Thericeinteractome,inwhichanetworkofprotein-...
Here, we developed an in vitro split luciferase complementation-based assay to speed up screening of PA–PB1 interaction inhibitors. By screening 10,000 compounds, we identified two PA–PB1 interaction inhibitors, R160792 and R151785, with potent and broad-spectrum antiviral activity against a ...
A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Reni...