Here, we report a cell-based assay for detecting SNAP-25-cleaving BoNTs by combining human induced Pluripotent Stem Cells (hiPSC)-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase. This assay is convenient, rapid, free-of-specialized antibodies, with a ...
Misawa N, Kafi A, Hattori M, Miura K, Masuda K, Ozawa T (2010) Rapid and high-sensitivity cell-based assays of protein−protein interactions using split click beetle luciferase complementation: an approach to the study of G-protein-coupled receptors. Anal Chem 82:2552–2560 Article CAS Go...
Luciferase assays were performed in quadruplicate starting from 4 cell cultures. Immunofluorescence was performed in duplicate from 2 separate cell cultures. All data are presented as means ± standard errors (SEs). The significance of differences between two groups was assessed using Student’s ...
A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Reni...
Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein pull-down, yeast two-hybrid (Y2H), co-immunoprecipitation (co-IP), and bimolecular fluorescence complementation (BiFC) assays. The recently established split-luciferase ...
(384- and 1536-well formats) CETSA platform that uses a split Nano Luciferase approach (SplitLuc CETSA). The broad applicability of the assay was demonstrated for diverse targets, and its performance was compared with independent biochemical and cell-based readouts using a set of well-...
Figure 4. Illustration of the measurement of the relative luciferase activity in Split-LUC imaging assays. 在Bio-protocol期刊查看原文,可以获取更多内容,包括摘要、背景、耗材试剂、仪器软件等详细信息。感兴趣的小伙伴,复制下方链接,一睹为快吧! 原文链接:Split-luciferase Complementation Imaging Assay to Study...
luciferase assay reagent, incubated at room temperature for 1 min and luminescence was subsequently read using a luminometer with 10 s integration time. All reported data are at least in duplicate and assays have been repeated on different days. Data from the reported luciferase assays were all ...
external measures unfeasible. Since the tumor cells expressed luciferase, we established a radiance threshold of ≥1010photons/s/cm²/sr as an upper surrogate limit; however, this was not attained in this study. Animals that experienced a 20% loss of their original body weight were euthanized....
Fluorogen-activating proteins (FAPs) are innovative fluorescent probes combining advantages of genetically-encoded proteins such as green fluorescent protein and externally added fluorogens that allow for highly tunable and on demand fluorescent signalin