Using doxorubicin-liposome-loaded microbubbles, we show that sonoprinting allows to deposit large amounts of doxorubicin-containing liposomes to the outer cell layers of the spheroids, followed by doxorubicin release into the deeper layers of the spheroids, resulting in a significant reduction in cell...
The resulting LTPT formulation was used for release studies after passing through a Sephadex-G25 column equilibrated with pH 7.4 phosphate buffer saline (PBS) or stored at 4 °C until needed. Liposomal Characterization Liposome particle size was analyzed by dynamic light scattering with the aid ...
Tumor spheroids is a 3D culture of cancer cells. This type of cell culture is a great tool for the evaluation of novel nanomedicine systems and in other areas of biomedical engineering. The main advantage over monolayer cell cultures is the biomimetic...
Article: Effects of hyaluronidase on doxorubicin penetration into squamous carcinoma multicellular tumor spheroids and its cell lethality
Leukocyte-mimetic drug delivery systemLiposomeTumor spheroidAs leukocytes can penetrate into deep regions of a tumor mass, leukocyte-mimetic liposomes (LM-Lipo) containing leukocyte membrane proteins are also expected to penetrate into tumors by exerting properties of those membrane proteins. The aim of...
by Nelly Vera 1,†, Stephanie Acuña-Gallardo 1,†, Felipe Grünenwald 1, Albano Caceres-Verschae 1, Ornella Realini 1, Rodrigo Acuña 2,3, Alvaro Lladser 4, Sebastián E. Illanes 1,5and Manuel Varas-Godoy 1,6,* 1 Laboratory of Reproductive Biology, Center for Biomedical Research...