introduced endothelial cell (EC) spheroids as a three-dimensional in vitro model resembling angiogenic responses and sprouting behavior [1]. As such, EC spheroids are capable of giving rise to capillary-like sprouts which are relatively close to the physiologically and genetically programmed arrangement...
Figure 3.Cell-Based 3D Models and Microfluidic Cancer-on-a-Chip (CoC) Models. Schematic representations of different cell based as well as microfluidic models describing the key steps and characteristics of each culture. (A) Fabrication of 3D (hetero)spheroids starting with (i) selection of cell...
In addition, VEGF already showed a beneficial effect on the viability and sprouting of HUVEC spheroids [34], [83], [84]. In these experimental conditions, at day 2, a thick layer of endothelial cells was observed around the fibronectin-rich cytokeratin-negative core (Fig. S9c). On the ...
We have therefore developed a complete suite of in vitro three-dimensional spheroid-based assays for measuring tumor growth, migration, invasion and tumor angiogenesis that, combined with new technologies of automated imaging and analysis, demonstrate applicability in relatively high-throughput formats. We...
As new treatment strategies continue to evolve, cell-based therapies have emerged as promising options for a number of diseases12,13. Although stem cells have beneficial effects, their clinical applications face many challenges, including extensive labor, high cost, and safety concerns. There are con...
Three-dimensional tumor spheroid-based functional assays: migration and invasion.(a)Tumor spheroid-based migration is illustrated in a cartoon: a single day 4 tumor spheroid was transferred to each well of extracellular matrix (ECM)-coated 96-well flat-bottomed plate using a multichannel pipette.(...
This paper reports on a microfluidic-based three-dimensional, cortical spheroid grafted on the vascular-network. Vascular network was formed by inducing angiogenic sprouting using concentration gradient-driven angiogenic factors in the microfluidic device. We investigate critical fact...
We have therefore developed a complete suite of in vitro three-dimensional spheroid-based assays for measuring tumor growth, migration, invasion and tumor angiogenesis that, combined with new technologies of automated imaging and analysis, demonstrate applicability in relatively high-throughput formats. We...
SproutingEndothelial cellsAngiogenic stimuliAngiogenesisVascularIn vitro models mimicking capillary sprouting are important tools to investigate the tumor angiogenesis, developmental blood vessel formation, and pathophysiological remodeling processes of the......
The endothelial cells are the main regulators of the angiogenic process, and thus the angiogenic outcome is assessed based on the effect on endothelial cell functions. Several in vitro and in vivo techniques have been developed to assess the effect of various factors on angiogenesis. Compared to ...