These crypts were then cultured in a medium containing critical growth factors (Wnt3a, R-spondin and Noggin) to enable the expansion of proliferative stem and progenitor cells. In doing so, small intestinal epithelial spheroids formed.Ray,K.Nature reviews. Gastroenterology & hepatology...
We established patient-derived spheroids (PDSs) and patient-derived organoids (PDOs) from PDCCs isolated from the malignant pleural effusion in 8 patients. The morphologies suggested that PDSs may be a model of local cancer extensions, while PDOs may be a model of distant cancer metastases. ...
using “native” immune cells can also be obtained from dissociated tumor samples cultured in Matrigel domes121or acoustically assembled spheroids122and are more easily cryopreserved. Nevertheless, due to the lack of immune-specific factors, immune cells infiltrating this type of PDTO exhibit a progres...
We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate ...
Mass spectrometry-based proteomics of 3D cell culture: a useful tool to validate culture of spheroids and organoids. SLAS Discov. 2022;27(3):167–74. Article CAS PubMed Google Scholar Brazovskaja A, Treutlein B, Camp JG. High-throughput single-cell transcriptomics on organoids. Curr Opin...
spheroids [62]. Because there were cells positive for both E-cadherin and Vimentin, it is likely that partial EMT occurs in spheroids, the PDX model and the original tumor [62,65]. Single cancer cell migration, also known as mesenchymal migration, is characterized by fibroblast-like morphology...
(e.g., hyaluronic acid31,32or alginate34), as well as synthetic matrices35,36,37,38,39have been developed as matrices for spheroid growth from cancer cell lines and organoid culture. These spheroids, however, often do not reflect the biology and the clinical spectrum of primary cancer cells...
Sarcoma Spheroids and Organoids-Promising Tools in the era of Personalized Medi- cine. Int J Mol Sci. 2018;19(2). 24. Gao S, Shen J, Hornicek F, Duan Z. Three-dimensional (3D) culture in sarcoma research and the clinical significance. Biofabrication. 2017;9(3):032003. 25. Haas RL...
W. et al. Profiling of PD-1 blockade using organotypic tumor spheroids. Cancer Discov. 8, 196–215 (2018). Article CAS PubMed Google Scholar Sbirkov, Y. et al. A colorectal cancer 3D bioprinting workflow as a platform for disease modeling and chemotherapeutic screening. Front. Bioeng. ...
In conclusion, clonal cells cultured on MEFs appeared to better preserve the undifferentiated state and stemness of CSCs, whereas spheroids under suspension culture condition showed signs of partial differentiation which could impact the purity, quantity, and growth microenvironment of CSCs, eventually ...