Research on cancer therapies has benefited from predictive tools capable of simulating treatment response and other disease characteristics in a personalized manner, in particular three-dimensional cell culture models. Such models include tumor-derived spheroids, multicellular spheroids including organotypic ...
Other 3D tumor cell culture approaches have subsequently emerged, including tumor explants obtained from slices of cancer tissue5, organotypic spheroids from patients’ tumor fragments cultured under nonadherent conditions6, tumorospheres generated from self-renewing tumor-initiating cells7,8, and tumor s...
Deep Annotation of Donated Chemical Probes (DCP) in Organotypic Human Liver Cultures and Patient-Derived Organoids from Tumor and Normal Colorectum Well-characterized small molecules are essential tools for studying the biology and therapeutic relevance of a target protein. However, many compounds repor...
et al. Organoid modeling of the tumor immune microenvironment. Cell 175, 1972–1988 (2018). Article CAS PubMed PubMed Central Google Scholar Jenkins, R. W. et al. Profiling of PD-1 blockade using organotypic tumor spheroids. Cancer Discov. 8, 196–215 (2018). Article CAS PubMed Google...
Patient-derived tumour organoids (PDOs) are highly advanced in vitro models for disease modelling, yet they lack vascularisation. To overcome this shortcoming, organoids can be inoculated onto the chorioallantoic membrane (CAM); the highly vascularised, not innervated extraembryonic membrane of fertil...
2dspheroids suspended in a medium using 3D matrix e.g.: agarose,2espheroids suspended in a medium using low attachment U bottom plates,2fspheroids in stirred systems, such as bioreactors,3aand3bPatient-derived tumouroids: Single cells derived from patient tumour samples and suspended in 3D ...
several experimental models of to study cancer development and progression including rats and mice as well as the in vitro culture models of cell line, 2D cell, induced pluripotent stem cell (iPSCs) lines, spheroids, 3D organoids, organotypic tissue slice cultures, patient-derived tumor xenografts...
Patient-derived xenograft mouse models can reflect inter-patient heterogeneity, but are expensive, time consuming, lack the presence of a functional immune system, and have the extra complexity of infiltrating mouse stroma into a tumour of human origin11,12. GEMMs contain a complex multicellular ...
targets for therapeutic intervention5. New promising in vitro models are being developed to address the deficiencies in the current systems. These include spheroids, organoids, organotypic tumor models, and ex vivo systems6. However, these systems still do not fully capture the complement of cancer...
Tumor-derived spheroids can develop from a single cell or cell aggregates. Culture supplements include growth factors, such as epidermal growth factor (EGF) and fibroblast growth factor (FGF). It is generally accepted that cell populations with stem cell-like properties will be functionally enriched...