Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques. 展开 ...
Advances in multicellular spheroids formation J. R. Soc. Interface, 14 (2017), 10.1098/rsif.2016.0877 Google Scholar [34] M. Zanoni, F. Piccinini, C. Arienti, A. Zamagni, S. Santi, R. Polico, A. Bevilacqua, A. Tesei 3D tumor spheroid models for in vitro therapeutic screening: a sys...
Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques.Ruei-Zhen Lin...
There is evidence that mitochondrial dysfunction precedes cartilage degradation and contributes to the death of chondrocytes [22]. Multiple factors have been identified as causes of mitochondrial impairment in OA, such as inflammation, ageing, infection, lack of nutrients, and genetic mutations [5]. O...
Multicellular co-cultures The liver is composed of various cells, including hepatocytes, which are the most abundant and mainly responsible for metabolism, biliary epithelial cells, hepatic stellate cells (HSCs) that store vitamin A, Kupffer cells (KCs) that are resident macrophages, and hepatic sinu...
3D cell culture methods — and multicellular tumorspheroids(MCTS), in particular — are quickly becoming a valuable tool for studying the tumor microenvironment incancer research. Cancer scientists are creating increasingly complex tumor models that more ac...
Multicellular co-cultures The liver is composed of various cells, including hepatocytes, which are the most abundant and mainly responsible for metabolism, biliary epithelial cells, hepatic stellate cells (HSCs) that store vitamin A, Kupffer cells (KCs) that are resident macrophages, and hepatic sinu...
K. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol. Bioeng. 83, 173–180 (2003). Article CAS Google Scholar Kelm, J. M. & Fussenegger, M. Microscale tissue engineering using gravity-enforced cell assembly. Trends ...
Moreover, with utilizing the micropatterning strategy, 3D microwell arrays can be obtained for generating multicellular spheroids. For instance, researchers have reported a case where micropatterning was used to yield a micro-coculture platform. In this platform, breast tumoroids were seeded inside micr...
[203], (2) continuous printing of polymeric bioink loaded with endothelial cells followed by polymer removal [96], (3) printing perfusable channels in a 3D construct for subsequent injection of a cell suspension into the empty channel [92], and (4) printing of multicellular spheroids [132]....