despite extensive scRNA-seq profiling across the brain, including cortex21,22,23. With recent advances in spatial transcriptomics techniques, such questions can now be addressed. Using multiplexed error-robust fluorescence in situ hybridization (MERFISH), an image-based method for spatially resolved sing...
The mammalian brain contains many specialized cells that develop from a thin sheet of neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of molecularly diverse cell types in the nervous system, but the lineage relationships b
Single-cell transcriptomics has been used to categorize cell types from many different regions of the mouse nervous system and increasingly in human and non-human primate brains2,12. The BRAIN Initiative Cell Census Network (BICCN) and the Human Cell Atlas (HCA) are representative community ...
Figure 1. Spatially Resolved Transcriptomics Profiles in Adult Mouse Brain (A) Sequential 10-μm coronal sections of AppNL-G-F and WT brains were collected at 3, 6, 12, and 18 months of age. The middle section was used for ST and the two adjacent sections for immunostaining. (B) Total...
In this study, we systematically evaluated six computational algorithms for cell type matching across four image-based spatial transcriptomics experimental protocols (MERFISH, smFISH, BaristaSeq, and ExSeq) conducted on the same mouse primary visual cortex (VISp) brain region. We find that many ...
Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease (AD) brain by spatial sequencing in mouse models, enabling the identification of unique genome-wide transcriptomic features associated with different spatial regions and pathological status. However, the dynamics of gen...
Spatial transcriptomics technologies developed in recent years can provide various information including tissue heterogeneity, which is fundamental in biological and medical research, and have been making significant breakthroughs. Single-cell RNA sequen
Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays. Cell. 2022;185(10):1777-92.e21. CAS PubMed Google Scholar Srivatsan SR, Regier MC, Barkan E, Franks JM, Packer JS, Grosjean P, et al. Embryo-scale, single-cell spatial transcriptomics. Science. ...
Spatial transcriptomics, commercialized as Visium Spatial Gene Expression, is a molecular profiling method that enables scientists to map whole transcriptome gene activity across an entire tissue section alongside H&E morphological imaging.The next evolution of this platform—powered by Visium CytAssist, ...
Spatial transcriptomics (ST) is a method that maps gene expression in tissue while preserving spatial information. It can reveal cellular heterogeneity, spatial organization and functional interactions in complex biological systems. ST can also complement and integrate with other omics methods to provide ...