This protocol describes a simple, convenient and sensitive single-nucleotide polymorphism (SNP) genotyping method using an optically amplifying cationic conjugated polymer and single base primer extension reaction. The fluorescence resonance energy transfer (FRET) efficiency between the conjugated polymer (PFP...
Progress in high throughput SNP genotyping methods - Tsuchihashi, Dracopoli - 2002 () Citation Context ...enic and causative of disease in the family. DISCUSSION Considerable technical developments in array design and assay development have led to methods allowing efficient high-throughput SNP ...
The application of genome-wide SNP genotyping methods in studies on livestock genomes Artur Gurgul, Ewelina Semik, Klaudia Pawlina, Tomasz Szmatoła, Igor Jasielczuk, Monika Bugno-Poniewierska $44.95 / €39.95 / £33.95 * Rent the article at a discount Rent now * Final gross...
Genotyping over 100,000SNPs on a pair of oligonucleotide arrays J. Nat Methods,2004,1(2):109- 111.5 CAVAN 13、AGH C R,CHAO S M,WANG S C,et al. Genome- widecomparative diversity uncovers multiple targets of selection for im-provement in hexaploid wheat landraces and cultivars J. Proc ...
To facilitate the utility of SNP-based genotyping, we developed a new method called target SNP-seq which combines the advantages of multiplex PCR amplification and high throughput sequencing. Compared with KASP, Microarrays, GBS and other SNP genotyping methods, target SNP-seq is flexible both in ...
Given the potential impact of SNPs on healthcare, the biotechnology industry has focussed urgently on the development of high-throughput methods for SNP genotyping. All genotyping methods are a mix and match of different allele discrimination and signal detection technologies and as such may represent...
In parallel, exome based genotyping methods have been evolved like TaqMan and Competitive Allele-Specific PCR (KASPar) assays [12], [13]. These protocols are based on the competitive and quantitative PCR amplification and scoring of alleles at a particular locus, respectively. Undoubtedly, these ...
The use of single nucleotide polymorphism (SNP) arrays to generate large SNP datasets for comparison purposes have recently become an attractive alternative to other genotyping methods. Although most SNP arrays were originally developed for domestic organisms, they can be effectively applied to wild rela...
Homogeneous allele-specific PCR in SNP genotyping Author information: (1)Roche Molecular Systems, Alameda, CA, USA. Sren Germer,R Higuchi - 《Methods Mol Biol》 被引量: 48发表: 2003年 [Methods in Molecular Biology] Single Nucleotide Polymorphisms Volume 578 || Allele-Specific PCR in SNP ...
After genotyping, the data obtained needs to be processed and analyzed. This involves quality control measures, such as filtering out low-quality SNPs or samples with a low call rate. Statistical methods are then applied to assess the genetic diversity within the population. Measures such as allel...