单核苷酸多态性(single nucleotide polymorphism,snp),主要是指在基因组水平上由单个核苷酸的变异所引起的dna序列多态性。它是人类可遗传的变异中最常见的一种。占所有已知多态性的90%以上。snp在人类基因组中广泛存在,平均每500~1000个碱基对中就有1个,估计其总数可达300万个甚至更多。 snp所表现的多态性只涉及到...
1.Single nucleotide polymorphism (SNP) is the molecular marker for the third generation human genome map.单核苷酸多态性(SNP)是第三代的人类基因组作图的分子标记,它指由单个碱基交换,插入或缺失,引起人群中出现两种有微小差异的DNA序列。 3)SNP[英][,es en 'pi:][美]['?s '?n 'pi]单核苷酸多态性...
F. (2003) Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput. Pharmacogenomics J. 3 , 77–96.Chen X, Sullivan PF (2003) Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput. Pharmacogenomics J 3:77–96...
(2013) Single nucleotide polymorphism genotyping using BeadChip microarrays. Curr. Protoc. Hum. Genet., Chapter 2, Unit 2 9.Lambert, G. et al. (2013) Single nucleotide polymorphism genotyping using BeadChip microarrays, Current protocols in human genetics / editorial board, Jonathan L. Haines .....
Table 1B. Single nucleotide polymorphism (SNP) genotype distribution, allele frequencies and mRNA expression SNP genotypingn (%)Allele frequencies ERCC1 C/C 11 (17.7) C (0.45) C/T 34 (54.8) T (0.55) T/T 17 (27.4) XPD 751 Lys/Lys 27 (43.5) Lys (0.66) Lys/Gln 28 (45.2) Gln ...
DNA dangling-end-induced colloidal stabilization of gold nanoparticles for colorimetric single-nucleotide polymorphism genotyping. A single-nucleotide polymorphism (SNP) detection method was developed by combining single-base primer extension and salt-induced aggregation of gold nanopa... Y Akiyama,H Shikag...
Many molecular genetic studies of human diseases involve determining the genotypes for single nucleotide polymorphisms. This chapter summarises a number of different techniques for the single nucleotide polymorphism genotyping which can be applied to MMP genes. The chapter also provides a protocol and tec...
Wang J, Lin M, Crenshaw A et al. High-throughput single nucleotide polymorphism genotyping using nanofluidic Dynamic Arrays.BMC Genomics2009;10 PubMedPubMed CentralGoogle Scholar Fluidigm SNP Genotyping User Guide Rev H1, PN 68000098. South San Francisco, CA: Fluidigm Corporation, 2011. ...
Objective:To establish an accurate and rapid single nucleotide polymorphism(SNP)genotyping method of Panax ginseng and P. 目的:建立快速、准确的单核苷酸多态性(SNP)测定法鉴别人参、西洋参及2种样本的混合物。2) single nucleotide polymorphism (SNP) 单核苷酸多态性(SNP) 1. Single nucleotide polymorphism...
Markers were developed based upon single nucleotide polymorphisms (SNPs), which are abundant and amenable to automated genotyping platforms. The focus of this research was SNP discovery in expressed porcine genes providing markers to develop the porcine/human comparative map. Locus specific amplification ...