Each predesigned TaqMan SNP Genotyping Assay is delivered in a single tube consisting of two differentially labeled allele-specific TaqMan MGB (minor groove binding) probes and a pair of PCR primers. For more information, please see the following link: https://www.thermofisher.com/us/en/home/...
Each TaqMan SNP Genotyping Assay includes two differentially labeled, allele-specific TaqMan MGB probes and a PCR primer pair that uniquely amplify and provide unmatched specificity for the allele of interest. Choose an assay from one of more than 17 million predesigned assays, or customize your own...
基因分型(Genotyping)又称为基因型分析(Genotypic assay),是利用生物学检测方法测定个体基因型(Genotype)的技术,使用技术包括聚合酶链反应(PCR)、基因测序、DNA片段分析等。基因分型目前已经成为很多工作的基础,比如基因功能研究、致病基因鉴定、基因型鉴定等。 直接法测序 SNP(single nucleotide polymorphism),即单核苷酸...
All custom assays use TaqMan minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All custom human SNP genotyping assays are functionally tested using 20 unique DNA samples to ensure assay amplification and s...
Applied Biosystems公司SNP研究完整解决方案_new Life Technologies公司SNP研究 完整解决方案
Conclusions The SNP genotyping assay and bioinformatic pipeline developed here present a valuable tool for increasing the power and decreasing the cost of many studies that depend on speed congenics. The assay is highly flexible and can be used for combinations of strains that are commonly used ...
3.DNA的扩增2× TaqMan Universal PCR Master Mix试剂盒与40× TaqMan SNP Genotyping Assays试剂盒联合使用。 (1)DNA、引物及探针用量DNA纯度:OD260/OD280=1.6~2.0,浓度1~20ng/pl。 引物和探针浓度: 引物/ Probe mix 贮存液(20×) 工作液(1×) 每反应用量 6-FAM Probe 4Hmol/L 0Onmol/L 5pmol/ ...
SNP GenotypingAssay Service is a con,dential and secure service,custom-ers per,orm theirown bioin,ormatics analysis prior tosubmission o,sequence ,orassaydesign A. Fr,(m)rk(r S),(ctio)Too,s SNPbrowser™So(tware)or Human SNPs
AQPTM基因分型系统又称等位基因特异的定量PCR基因分型系统(Allele-SpecificQuantitativePCR based genotyping assay, AQP),是一种PCR扩增技术与定量PCR技术结合形成的基因分型系统。该系统采用终点法读取荧光值,能够对SNP、插入或缺失变异位点进行检测,具有很大的灵活性,检测成本低,特别适用于大批量筛选项目,例如植物育种...
3.DNA的扩增2× TaqMan Universal PCR Master Mix试剂盒与40× TaqMan SNP Genotyping Assays试剂盒联合使用。 (1)DNA、引物及探针用量DNA纯度:OD260/OD280=1.6~2.0,浓度1~20ng/pl。 引物和探针浓度: 引物/ Probe mix 贮存液(20×) 工作液(1×) 每反应用量 6-FAM Probe 4Hmol/L 0Onmol/L 5pmol/ ...