KASPspoon: an in vitro and in silico PCR analysis tool for high-throughput SNP genotyping KASPspoon compares physical and genetic maps, and reports the primer set genome coverage for PCR-walking. KASPspoon could be used to design KASP assay ... AM Alsamman,SD Ibrahim,A Hamwieh - 《Bioinfo...
TaqMan SNP Genotyping Kit ASL(Allele Specific Ligation) TaqMan PCR Genotyping Kit是自主研发的创新型基因分型试剂盒,使用该技术方案可快速高效的进行SNP、插入缺失等基因分型试验。 相比于其它单位点SNP分型技术(Thermo Fisher的MGB-qPCR探针法、LGC的KASP法、ARMS-qPCR法),ASL技术方案,结果更为准确可靠、分型清...
Semagn K, Babu R, Hearne S等. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): an overview of the technology and its application in crop improvement. Mol Breeding. 2014;33:1-14.杨青青, 唐家琪, 等. KASP标记技术在主要农作物中的应用及展望. 生...
Genotyping-by-sequencing (GBS) based single nucleotide polymorphisms (SNP) enabled Kompetitive allele specific PCR (KASP) marker development in mutant Rubus genotypesdoi:10.1016/j.ejbt.2018.08.001Ryu, JaihyunkKim, Woon JiIm, JuhyunKim, Sang Hun...
using deep transcriptome sequencing of eight diversePisumaccessions were used for genotyping in five RIL populations using an Illumina GoldenGate assay. The first high-density pea SNP map defining all seven linkage groups was generated by integrating with previously published anchor markers. Syntenic ...
KASP genotyping, but need further purification to ensure their quality for RAD-seq library construction. Thus, after extracting DNA of 31 accessions used for RAD-seq, we utilized the QIAGEN kit (DNeasy Blood & Tissue Kit; Qiagen,https://www.qiagen.com/, Hilden, Germany) to purify these ...
Superb accuracy(>99.8%) and performance(>90% SNP and indel assay conversion rate) 拥有超高的准确率和稳定性 Our genotyping and extraction technology is key to helpingscientists understandthe genetics of crop。 我们的分子型检测和DNA提取技术是帮助科学家们研究作物遗传 的关键核心。 We work with all ...
Our KASP genotyping panels are pre-validated, ready-to-use SNP genotyping assays for DNA samples from humans, rats, mice, and more! Click here!
Akhunov, E., Nicolet, C. Dvorak, J. 2009. Singlenucleotidepolymorphism genotyping in polyploid wheat with the Illumina Golden Gate assay. Theor. Appl. Genet. 119: 507-517. Durstewitz, G., Polley, A., Plieske, J., Luerssen, H., Graner, E.M., Wieseke, R., Ganal, M.W. 2010....
Here, we report the development of a genome-wide set of kompetitive allele specific PCR (KASP) markers for marker-assisted recurrent selection (MARS) in the tetraploid minor crop perilla. To find locus-specific SNP markers across the perilla genome, we used genotyping-by-sequencing (GBS) to ...