试剂组成:包含 3 种组分,感兴趣 SNP 的测试DNA;KASP Assay Mix 包含两种不同的、具有独特末端序列的等位基因特异性竞争正向引物和一种反向引物;KASP Master mix 包含 FRET 盒与 Taq 聚合酶并保存于优化的缓冲液中。 实验主要分为三步: 第一步 PCR 循环-模板变性和组分退火:其中一种等位基因特异性引物和通用反...
SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population The subsequent genotyping of a subset of 1000 SNPs, chosen for their mapping positions using a KASP assay, showed that almost all generated SNPs are ... G Boutet,S Alves Carv...
SNP基因分型:KASP分析 相关领域 基因分型 SNP基因分型 单核苷酸多态性 索引 SNP公司 计算生物学 生物 PCR变异 遗传学 基因型 分子反转探针 鉴定(生物学) 等位基因 基因 植物 网址 https://doi.org/10.1007/978-1-4939-0446-4_7 DOI 10.1007/978-1-4939-0446-4_7 复制 doi ...
Single nucleotide polymorphism (SNP) discovery through genotyping-by-sequencing (GBS) and genetic characterization of Dendrobium mutants and cultivarsDendrobiumMutantKASPGBSSNPDendrobiwn plants are the most important orchids used in horticultural industries and oriental medicine. Recently, several Dendrobium ...
5. He C, Holme J, Anthony J. SNP genotyping: the KASP assay. Methods Mol Biol. 2014;1145:75-86. doi: 10.1007/978-1-4939-0446-4_7. PMID: 24816661. 6. Ren R, Xu J, Zhang M, Liu G, Yao X, Zhu L, Hou Q. Identification and Molecular Mapping of a Gummy Stem Blight Resistanc...
in alignment of the allele specific primers to sequence regions in all three sub-genomes and sometimes also to paralogous regions. Therefore, the specificity of the KASP assay depends mainly on selecting common primer binding sites with one or more nucleotide differences at 3`end of the primer ...
Genotyping-by-sequencing (GBS) based single nucleotide polymorphisms (SNP) enabled Kompetitive allele specific PCR (KASP) marker development in mutant Rubus genotypesdoi:10.1016/j.ejbt.2018.08.001Ryu, JaihyunkKim, Woon JiIm, JuhyunKim, Sang Hun...
Semagn K, Babu R, Hearne S等. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): an overview of the technology and its application in crop improvement. Mol Breeding. 2014;33:1-14.杨青青, 唐家琪, 等. KASP标记技术在主要农作物中的应用及展望. ...
The continuous progress in high-throughput sequencing has facilitated the development of some high-throughput SNP genotyping methods, such as the Gene Chip microarray or the KASP platform (competitive allele-specific PCR)16. While chip-based platforms require enormous amounts of time for SNP assay ...
The primer sequences can be found in Supplementary Table 1, Additional File 2. The PACE® assay was performed using PACE® 2.0 Genotyping Master Mix with a low Rox concentration according to the manufacturer's instructions. For a 384-well plate, 2.5 µL of DNA (15 ng) and 2.5 µ...