竞争性等位基因特异性PCR(kompetitive allele-specific PCR,KASP)是一种基于已知SNP的终点荧光基因分型技术,可在DNA样品中对特定位点上的SNP和InDel进行精准的双等位基因检测。 KASP反应体系中包含DNA模板、2个通用荧光探针FAM和HEX、2个通用淬灭探针、2个正向引物、1个反向引物、ROX阴性参比染料和Taq酶等。 KASP基因...
Analysis of DNA polymorphism in historic barley material: validation of the KASP SNP genotyping platform. Taxon 62 : 779–789.Lister D L, Jones H, Jones M K, O'Sullivan D M, Cockram J. Analysis of DNA polymorphism in ancient barley herbarium ma- terial: validation of the KASP SNP...
APPLICATION NOTE 在 SpectraMax 微孔读板机上利用 KASP 基因分型技术检测 SNPs Young Mee Yoon | Field Applications Scientist | Molecular Devices 前言 基因分型是一种在不同个体间检测他们的 DNA 序列以分析遗传差异的过...
Semagn K, Babu R, Hearne S等. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): an overview of the technology and its application in crop improvement. Mol Breeding. 2014;33:1-14.杨青青, 唐家琪, 等. KASP标记技术在主要农作物中的应用及展望. 生...
SNP genotyping: the KASP assay. In: Fleury D, Whitford R, editors. Crop Breeding. New York: Springer New York; 2014. p. 75-86.He, C., Holme, J., and Anthony, J. (2014). SNP genotyping: the KASP assay. Methods Mol. Biol. 1145: 75-86....
镁伽全自动KASP基因分型和NGS建库系统具备两大功能:1、KASP基因分型:完成从植物碱煮法核酸提取到基因分型结果报告出具的全流程自动化操作,无需人工介入,为有效SNP标记验证、功能标记开发提供支持;2、NGS建库:完成从种子研磨、磁珠法核酸提取到文库构建的全流程自动化操作,输出样本直接用于测序分析。自动化建库流程包括...
SNP within 300 kb of both flanks of target genes or loci for related traits. KASP markers were developed according to the differences of SNPs, and the close link between KASP markers and the target characteristics was confirmed by genotyping.【Result】 The developed KASP molecular markers for ...
沉淀的分型 1.自由沉淀。悬浮颗粒的浓度低,在沉淀过程中呈离散状态,互不粘合,不改变颗粒的形状、尺寸及密度,各自完成独立的沉淀过程。这种类型多表现在沉砂池、初沉池初期。 2.絮凝沉淀。悬浮颗粒的浓度比较高(50~500mg/L),在沉淀过程中能发生凝聚或絮凝作用,使悬浮颗粒互相碰撞凝结,颗粒质量逐渐增加,沉降速度...
1 SNP comparison of genotyping and grain selenium content 20 期 魏广辉等:人工合成小麦 SHW-L1 高硒含量 KASP 分子标记开发及其应用 4107 不存在,因此,要从初筛选的标记中做二次筛选,只 需选出基因型聚类较好的标记即可,最终选出 5 个标 记用于开发籽粒硒含量相关的 KASP 分子标记(表 3). 根据 5 个...
Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement Single nucleotide polymorphism (SNP) data can be obtained using one of the numerous uniplex or multiplex SNP genotyping platforms that combine a ...