SNP genotyping: the KASP assay. In: Fleury D, Whitford R, editors. Crop Breeding. New York: Springer New York; 2014. p. 75-86.He, C., Holme, J., and Anthony, J. (2014). SNP genotyping: the KASP assay. Methods Mol. Biol. 1145: 75-86....
试剂组成:包含 3 种组分,感兴趣 SNP 的测试DNA;KASP Assay Mix 包含两种不同的、具有独特末端序列的等位基因特异性竞争正向引物和一种反向引物;KASP Master mix 包含 FRET 盒与 Taq 聚合酶并保存于优化的缓冲液中。 实验主要分为三步: 第一步 PCR 循环-模板变性和组分退火:其中一种等位基因特异性引物和通用反...
试剂组成:包含 3 种组分,感兴趣 SNP 的测试DNA;KASP Assay Mix 包含两种不同的、具有独特末端序列的等位基因特异性竞争正向引物和一种反向引物;KASP Master mix 包含 FRET 盒与 Taq 聚合酶并保存于优化的缓冲液中。 实验主要分为三步: 第一步 PCR 循环-模板变性和组分退火:其中一种等位基因特异性引物和通用反...
这种类型多表现在沉砂池、初沉池初期。 2.絮凝沉淀。悬浮颗粒的浓度比较高(50~500mg/L),在沉淀过程中能发生凝聚或絮凝作用,使悬浮颗粒互相碰撞凝结,颗粒质量逐渐增加,沉降速度
The primer sequences can be found in Supplementary Table 1, Additional File 2. The PACE® assay was performed using PACE® 2.0 Genotyping Master Mix with a low Rox concentration according to the manufacturer's instructions. For a 384-well plate, 2.5 µL of DNA (15 ng) and 2.5 µ...
Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations. Human Mutation, 2005, 2(5): 404-414. [12] GUNDERSON K L, STEEMERS F J, LEE G, MENDOZA L G, CHEE M S. A genome-wide scalable SNP genotyping assay using microarray technology. ...
Cost reduction is usually fulfilled by compiling a highly effective marker panel containing only dozens to hundreds of SNPs that are available for high-throughput genotyping platforms, such as Douglas ArrayTape and LGC Omega-F equipment, using the KASP genotyping assay. These systems allow users to...
用途:ASL(Allele Specific Ligation) TaqMan PCR Genotyping Kit是自主研发的创新型基因分型试剂盒,使用该技术方案可快速高效的进行SNP、插入缺失等基因分型试验。 相比于其它单位点SNP分型技术(Thermo Fisher的MGB-qPCR探针法、LGC的KASP法、ARMS-qPCR法),HaiGene的ASL技术方案,结果更为准确可靠、分型清晰。通常在3...
Superb accuracy(>99.8%) and performance(>90% SNP and indel assay conversion rate) 拥有超高的准确率和稳定性 Our genotyping and extraction technology is key to helpingscientists understandthe genetics of crop。 我们的分子型检测和DNA提取技术是帮助科学家们研究作物遗传 的关键核心。 We work with all ...
Single nucleotide polymorphism genotyping in polyploid wheat with the Illumina Golden Gate assay. Theor. Appl. Genet. 119: 507-517. Durstewitz, G., Polley, A., Plieske, J., Luerssen, H., Graner, E.M., Wieseke, R., Ganal, M.W. 2010. SNP discovery by amplicon sequencing and ...